▎ 摘　　要

NOVELTY - Detection of mercury ions involves adding partially double-stranded bisamino-poly-T sequence and activated graphene oxide to phosphate-buffered saline, reacting, centrifuging, mixing the supernatant with phosphate-buffered saline, mixing, measuring fluorescence intensity, adding divalent mercury ions, leaving the mixture still, detecting fluorescence intensity, analyzing mercury ion concentration by change in fluorescence intensity, determining sensitivity of the sensor, adding Yangtze river water, and testing mercury ion concentration. USE - Detection of mercury ions. ADVANTAGE - The method enables detection of mercury ions by effectively forming pair of T sequence, with improved signal-to-noise ratio. DETAILED DESCRIPTION - Detection of mercury ions involves preparing a mixture comprising 50 mM N-hydroxysuccinimide and 200 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, mixing the mixture with pure water and graphene oxide in volume ratio of 1:1:2, processing at room temperature for 30 minutes, obtaining activated graphene oxide, adding partially double-stranded bisamino-poly-T sequence and 10 mu g activated graphene oxide to 10 mM phosphate-buffered saline, reacting at 40 degrees C, centrifuging, mixing the supernatant with phosphate-buffered saline, mixing, measuring fluorescence intensity, adding divalent mercury ions to resultant liquid, mixing, leaving the mixture still for 20 minutes, detecting fluorescence intensity, analyzing mercury ion concentration by change in fluorescence intensity, determining sensitivity of the sensor, adding Yangtze river water, and testing mercury ion concentration.