▎ 摘　　要

NOVELTY - Electrochemical detection of endocrine disruptors based on enzyme nanoreactor involves: (1) mixing functionalized silicon dioxide with different diameters as a template with graphene oxide through hydrophobic-hydrophobic effect to obtain a composite material; (2) freeze-drying the material -20 degrees C, then calcining and washing with hydrofluoric acid to obtain a graphene foam; (3) dispersing the foam in water, carrying out functional modification on the foam by adding dopamine hydrochloride solution and adding graphene foam to dopamine hydrochloride solution to obtain porous nitrogen-doped graphene foam (PNGF) nanocomposite material; and (4) putting a certain amount of PNGF nanocomposite material into a centrifugal tube as enzyme nanoreactor, then adding active enzyme, uniformly mixing the mixture on oscillator and placing on glassy carbon electrode, taking three-electrode system and adding endocrine disrupter to electrolyte every time through a timing-current method. USE - The method is useful for electrochemical detection of endocrine disruptors based on an enzyme nanoreactor. ADVANTAGE - The method allows for rapid electrochemical detection of endocrine disruptors based on an enzyme nanoreactor with high sensitivity and accuracy and good stability. DETAILED DESCRIPTION - The step (1) involves dispersing graphite oxide powder in water, carrying out ultrasonic treatment for 4 hours, then centrifuging for 30 minutes under the condition of 3000 rpm to obtain a graphene oxide aqueous solution with a concentration of 0.5 mg/ml and mixing functionalized silicon dioxide and graphene oxide aqueous solution at room temperature for 12 hours to obtain a composite material. The calcining step in step (2) is carried out in an inert gas. The step (4) involves taking three-electrode system including Peltier electrode as auxiliary electrode, saturated calomel electrode as reference electrode and glassy carbon electrode as working electrode, where the concentration of glassy carbon electrode is 0.1 M, and the phosphate buffer solution having pH of 7 serves as electrolyte.