▎ 摘　　要

NOVELTY - Preparing a composite scaffold artificially simulating a bone marrow microenvironment involves providing three-dimensional carbon materials, reacting methacrylic anhydride with gelatin to prepare methacrylic anhydride gelatin, and modifying hyaluronic acid by cross-linking agent to obtain thiolated hyaluronic acid. The mixed reaction system comprising three-dimensional carbon material, methacrylic anhydride gelatin, thiolated hyaluronic acid, and photoinitiator is subjected to a cross-linking reaction under ultraviolet light irradiation to prepare a composite scaffold. USE - Method for preparing a composite scaffold artificially simulating a bone marrow microenvironment for an in vitro culturing of cells for non-medical purposes or in regulating cells (claimed). ADVANTAGE - The method prepares composite scaffold artificially simulating bone marrow microenvironment, which chemically bonds soluble growth factor and small molecule drug into three-dimensional scaffold, adds stromal cell to co-culture according to cell interaction in bone marrow microenvironment in the body to realize free combination of various culture systems, and participates in stem cell adhesion, migration, growth and other behaviors. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: a composite scaffold artificially simulating a bone marrow microenvironment comprises three-dimensional carbon material as a hard material and a hydrogel material as a soft material, where hydrogel material precursor solution is filled in voids of three-dimensional carbon material, and precursor solution is in-situ cross-linked, and combined with three-dimensional carbon material to form a composite scaffold, where composite scaffold has a three-dimensional pore network structure, and elastic modulus of the three-dimensional carbon material is 35-45 KPa, and elastic modulus of the hydrogel material is 0.5-8 KPa, and pore diameter of the pores contained in the composite scaffold is 20-100μm; and a method for culturing cell by preparing three-dimensional carbon material, methacrylic anhydride gelatin, mercapto hyaluronic acid, and photoinitiator; seeding hematopoietic stem cells on the three-dimensional carbon material, mixing it with the methacrylic anhydride gelatin, thiolated hyaluronic acid, photoinitiator, phosphate buffer, growth factors, and stromal cells, and cross-linking it to form hematopoietic stem cells, where three-dimensional co-culture system realizes the regulation of hematopoietic stem cells.