▎ 摘　　要

NOVELTY - The SERS substrate (100) has a substrate base (200) and a metal insulator metal (MIM) nanostructures (300) arranged on the substrate base, where an average distance between the MIM nanostructures arranged on the substrate base is from about 1 nm to about 10 nm, where each of the MIM nanostructures comprises metal or metal oxide layers (310) and insulator layers (320). The metal or metal oxide layers has an average thickness from about 20 nm to about 60 nm and each of the insulator layers has an average thickness from about 5 nm to about 10 nm. The substrate base is flexible and comprises an elastomer, the elastomer comprising a flexible polymer, silicone, polysiloxane, latex, or combinations. The metal or metal oxide layers comprise gold, silver, copper, aluminum, or alloys, Al/Ge doped zinc oxides, heavily doped indium tin oxides, metal nitrides, graphene, molybdenum disulfide, tungsten disulfide, or combinations. USE - SERS substrate for detecting pathogen such as SARS-CoV-2. ADVANTAGE - The surface-enhanced Raman spectroscopy substrate provides rapid results in point-of-care settings with minimal sample preparation, and provides highly sensitive and cost-efficient platforms for the detection of viruses and pathogens, such as SARS-CoV-2. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: a SERS biosensor; a method for making a surface-enhanced Raman spectroscopy SERS substrate; and a method of detecting pathogen using a SERS substrate. DESCRIPTION OF DRAWING(S) - The drawing shows a sectional view of the SERS substrate. 100SERS substrate 200Substrate base 300MIM nanostructure 310Metal oxide layer 320Insulator layer