• 专利标题:   Performing rapid detection method for myocardial infarction based on nano-gold-graphene quantum dots, comprises adding potassium oxide to the gold nanoparticle solution and mixing to get the first mixed solution.
  • 专利号:   CN111273022-A
  • 发明人:   HE B, GUO X, XUE X, ZHI S, LI H, HONG T, ZHOU M, WEI Y
  • 专利权人:   HE B, WANG X
  • 国际专利分类:   G01N021/64, G01N033/533, G01N033/68
  • 专利详细信息:   CN111273022-A 12 Jun 2020 G01N-033/68 202057 Pages: 10 Chinese
  • 申请详细信息:   CN111273022-A CN10081155 06 Feb 2020
  • 优先权号:   CN10081155

▎ 摘  要

NOVELTY - Performing rapid detection method for myocardial infarction based on nano-gold-graphene quantum dots, comprises adding potassium oxide to the gold nanoparticle solution and mixing to get the first mixed solution. Add troponin antibody to the first mixed solution, stir well to obtain the second mixed solution. Add the first concentration of bovine serum albumin solution to the second mixed solution, stir well to obtain a troponin detection solution. Add serum samples of different preset concentrations of troponin to the troponin detection solution. Add graphene quantum dots to the troponin detection solution after completing to obtain a fluorescent detection solution with different preset concentrations of troponin. USE - Method for performing rapid detection method for myocardial infarction based on nano-gold-graphene quantum dots. ADVANTAGE - The method enables to perform rapid detection method for myocardial infarction based on nano-gold-graphene quantum dots, which quickly and accurately detect the concentration of troponin, so as to quickly detect the concentration of troponin in the serum sample to be tested. DETAILED DESCRIPTION - Performing rapid detection method for myocardial infarction based on nano-gold-graphene quantum dots, comprises adding potassium oxide to the gold nanoparticle solution and mixing to get the first mixed solution. Add troponin antibody to the first mixed solution, stir well to obtain the second mixed solution. Add the first concentration of bovine serum albumin solution to the second mixed solution, stir well to obtain a troponin detection solution. Add serum samples of different preset concentrations of troponin to the troponin detection solution. Add graphene quantum dots to the troponin detection solution after completing to obtain a fluorescent detection solution with different preset concentrations of troponin. Excite the fluorescence detection solution containing different preset concentrations of troponin to obtain standard photoluminescence spectra corresponding to different preset concentrations of troponin. Add the actual serum sample to be tested to the troponin detection solution prepared by the method. Add graphene quantum dots to the detection solution after completing to obtain the actual fluorescence detection solution. Excite the actual fluorescence detection solution to obtain the photoluminescence spectrum of the actual serum sample to be tested, and according to the fluorescence intensity in the photoluminescence spectrum of the actual serum sample to be measured, corresponding to the relationship between the troponin concentration and the fluorescence intensity, calculate the troponin concentration in the actual serum sample to be tested.