▎ 摘　　要

NOVELTY - Preparing graphene oxide modified by chloroacetic acid that inhibits insulin amyloid fibrosis involves adding graphene oxide into distilled water, and then sonicating in an ultrasonic device for 1 to 3 hours to prepare a graphene oxide suspension. The graphene oxide suspension is placed in an ice-water bath, sodium hydroxide (NaOH) is added to the suspension and stirred at 250-300 revolutions per minute (rpm) for 10-20 minutes (min), chloroacetic acid is added and stirred at 250-300rpm for 10-20min, sonicated for 2-3 hours (h), then stirred at 25-30°C and 250-300rpm for 12-24h. The suspended particles are washed with deionized water and dried in vacuum to obtain graphene oxide modified with chloroacetic acid. 30 mg of graphene oxide, 1.5 to 5.0 g of NaOH and 2.0 to 6.0 g of chloroacetic acid are added for every 50 to 100 mL of distilled water. USE - Method for preparing graphene oxide modified by chloroacetic acid that inhibits insulin amyloid fibrosis for inhibiting insulin amyloid fibrosis (claimed). ADVANTAGE - The method enables to prepare graphene oxide modified by chloroacetic acid that inhibits insulin amyloid fibrosis, which is limits the flexibility of insulin molecule to undergo conformational transition, reduces the exposure of its hydrophobic core, and occupies its active extension site, thus inhibiting insulin amyloid fibrosis, has excellent performance, and has a simple preparation process. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for use of graphene oxide modified by chloroacetic acid that inhibits insulin amyloid fibrosis is claimed for inhibiting insulin amyloid fibrosis.