▎ 摘　　要

NOVELTY - An electrochemical detection method involves preparing a working electrode by modifying a gold electrode with analyte specific recognition of DNA probes, drawing standard curve by carrying out reaction on working electrodes and different standard sample solution to obtain standard sample solution-processed working electrode. The standard sample solution-processed working electrode is incubated with reduced graphene oxide to obtain reduced graphene oxide loaded working electrode. The obtained electrode is subjected to silver deposition reaction with solution containing silver ions. USE - Electrochemical detection method for catalytic silver deposition of reduced graphene oxide. ADVANTAGE - The method enables catalytic silver deposition of reduced graphene oxide with better safety, in a simple and easy manner. DETAILED DESCRIPTION - An electrochemical detection method involves preparing a working electrode by modifying a gold electrode with analyte specific recognition of DNA probes, drawing standard curve by carrying out reaction on working electrodes and different standard sample solution to obtain standard sample solution-processed working electrode. The standard sample solution-processed working electrode is incubated with reduced graphene oxide to obtain reduced graphene oxide loaded working electrode. The obtained electrode is subjected to silver deposition reaction with solution containing silver ions to obtain elemental silver deposited reduced graphene oxide working electrode. The electrode is processed with solution containing nitrate ion, followed by using stripping voltammetry determination of the current value of elemental silver using linear sweep voltammetry, taking specific activity of the standard samples as horizontal axis, and measured current value of elemental silver as vertical axis to draw standard curve samples to measure specific activity of the samples, taking test sample solution, and reacting the test sample solution with the obtained working electrode. The obtained electrode is reacted with reduced graphene oxide to obtain reduced graphene oxide loaded working electrode, which is subjected to silver deposition reaction carried out using silver ion-containing solution and solution containing the nitrate ion to obtain elemental silver-deposited working electrode, followed by using stripping voltammetry determination of the current value of elemental silver using linear sweep voltammetry, and calculating specific activity of the sample solution in the test sample according to standard curve, where the different standard sample solution comprises DNA probes of known specific activity to be detected and a substance capable of specifically recognizing the analyte to be cut into oligonucleotides single stranded substance, in each sample solution, and the DNA probe of the different standard sample solution specifically recognized in each of the analytes and oligonucleotides single stranded substance are equal, the sample solution comprises DNA probes of unknown specific activity to be detected and a substance capable of specifically recognizing the analyte to be cut into oligonucleotides single stranded substance, the content of the DNA probes of unknown specific activity to be detected and a substance capable of specifically recognizing the analyte to be cut into oligonucleotides single stranded substance in the test sample solution is equal to the content of the DNA probes of unknown specific activity to be detected and a substance capable of specifically recognizing the analyte to be cut into oligonucleotides single stranded substance in different standard solution.