▎ 摘　　要

NOVELTY - Preparing graphene oxide-polyamidoamine (PAMAM)-diethylenetriamine pentaacetic acid (DTPA)-gadolinium (Gd)-prostate cancer stem cell antigen (PSCA) antibody multi-functional material, comprises: (a) linking PAMAM on the surface of the nano-graphene oxide to obtain graphene oxide (GO)-PAMAM nanomaterial; (b) dissolving the nanomaterial in an organic solvent, adding triethylamine and DTPA dianhydride, continuously reacting and collecting precipitate; (c) mixing with soluble gadolinium (Gd) salt, collecting precipitate and dialyzing; and (d) reacting with PSCA-antibody and washing. USE - The (GO-PAMAM-(DTPA-Gd)-PSCA antibody) multifunctional material is useful in preparing contrast agent of MRI, and preparing drug carrier for treating prostate cancer. The (GO-PAMAM-(DTPA-Gd)-PSCA antibody-doxorubicin hydrochloride) multifunctional material is useful for treating prostate cancer (all claimed). The (GO-PAMAM-(DTPA-Gd)-PSCA antibody) multifunctional material is useful as a good T1 contrast agent. ADVANTAGE - The material has excellent biocompatibility and stability, and good prostate cancer cells targeting ability, contains doxorubicin hydrochloride adhered on the GO-PAMAM-(DTPA-Gd)-PSCA antibody material via the pi-pi bond, has large drug-loading amount and remarkable targeting effect, hence the material has functions of targeted drug delivery and MRI. DETAILED DESCRIPTION - Preparing graphene oxide-polyamidoamine (PAMAM)-diethylenetriamine pentaacetic acid (DTPA)-gadolinium (Gd)-prostate cancer stem cell antigen (PSCA) antibody multi-functional material, comprises: (a) linking PAMAM on the surface of the nano-graphene oxide: dispersing nano-graphene oxide in alcohol and activating, and mixing and stirring with the alcohol solution of the third generation PAMAM dendrimer using trimesic acid as an inner core for 18-30 hours to obtain graphene oxide (GO)-PAMAM nanomaterial, where the weight ratio of the nano-graphene oxide and the third generation PAMAM dendrimer is 1:20-40; (b) dissolving the GO-PAMAM nanomaterial in an organic solvent, adding triethylamine and DTPA dianhydride, heating at 70-85 degrees C for 20-60 minutes, continuously reacting for 10-18 hours after stopping the heating process, and collecting the resulting precipitate to wash to obtain GO-PAMAM-DTPA nanomaterial, where the weight ratio of the GO-PAMAM nanomaterial and the DPTA dianhydride is 4:1-8:1; (c) mixing the GO-PAMAM-DTPA nanomaterial with soluble gadolinium (Gd) salt, then incubating at 35-50 degrees C for 10-15 hours under the condition of absence of air, collecting precipitate, and dialyzing in water for 12-30 hours to obtain GO-PAMAM-(DTPA-Gd) nanomaterial, where the dosage ratio of the gadolinium element and the GO-PAMAM-DTPA nanomaterial is 0.002-0.01 mmol/mg; and (d) linking anti-prostate cancer stem cell antigen (PSCA) antibody: processing the GO-PAMAM-(DTPA-Gd) nanomaterial using glutaraldehyde, then reacting with anti-PSCA-antibody in phosphate buffer solution of 7.2-7.5 pH value at 0-6 degrees C for 10-16 hours, and washing, where the weight ratio of GO-PAMAM-(DTPA-Gd)nanomaterial and anti-PSCA-antibody is 10000:1-10000:5. INDEPENDENT CLAIMS are also included for: (1) a graphene oxide-PAMAM-(DTPA-Gd)-PSCA antibody multi-functional material, prepared by the method; and (2) a graphene oxide-PAMAM-(DTPA-Gd)-PSCA antibody-doxorubicin hydrochloride multi-functional material, prepared by stirring and reacting the GO-PAMAM-(DTPA-Gd)-PSCA antibody multi-functional material with phosphate buffer solution (pH value as 7.2-7.5) containing doxorubicin hydrochloride for 16-30 hours under the dark conditions, where the weight ratio of the doxorubicin hydrochloride and the GO-PAMAM-(DTPA-Gd)-PSCA antibody multi-functional material is 1:1-1:2.