▎ 摘　　要

NOVELTY - Method for preparing magnetic nanocarrier material, involves (a) performing preparation and surface modification of iron(II,III) oxide magnetic nanoparticles, (b) preparing graphene oxide, and (c) preparing magnetic iron(II,III) oxide/graphene oxide nanocarrier material by ultrasonically treating the graphene oxide suspension for 0.5-1.5 hours, then adding magnetic iron(II,III) oxide nanoparticles to the suspension, and further ultrasonically treating the mixture for 1 hour, and after reacting at 60degrees Celsius for 1 hour under mechanical stirring, obtaining the iron(II,III) oxide/graphene oxide composite material by magnetic separation, where the mass ratio of graphene oxide suspension and magnetic iron(II,III) oxide nanoparticles is (20-35):1. USE - The method is used for preparing magnetic nanocarrier material, which is used for reducing the side effects of epirubicin during tumor treatment. ADVANTAGE - The method loads epirubicin on the magnetic iron(II,III) oxide/graphene nanocarrier material, and its targeting effect can make epirubicin directionally concentrate and release to specific tumor sites, thus greatly reducing its toxic and side effects. The nanocarrier material has high magnetic saturation, high stability, low cytotoxicity and low production cost. DETAILED DESCRIPTION - Method for preparing magnetic nanocarrier material, involves (a) performing preparation and surface modification of iron(II,III) oxide magnetic nanoparticles by preparing deionized water and dimethylformamide in a volume ratio of 1:(3-5) into a dimethylformamide solution at room temperature, dissolving ferric chloride hexahydrate and 1,4-phthalic acid in the dimethylformamide solution, uniformly stirring to form a uniform solution, adding 2.5-3.5 %mass silane coupling agent KH550, uniformly stirring, dripping urea into the uniform solution at 1-300 drops/minute, continuously stirring during the dripping process, stopping the dripping process when the pH of solution reaches 8-9.5 and ultrasonically mixing uniformly, sealing the mixed solution, performing hydrothermal treatment, maintaining the temperature at 160degrees Celsius for 2-5 hours, cooling to room temperature, collecting the precipitate by centrifugation at 1500-3000 rpm for 15-30 minutes, washing the intermediate samples collected by centrifugation multiple times with dimethylformamide and absolute ethanol until neutral, drying, taking out, finely grinding and sealing for later use, (b) preparing graphene oxide by uniformly mixing graphite powder and potassium nitrate in a mass ratio of 2:1 at room temperature, adding to 98 %mass concentrated sulfuric acid solution that has been cooled to 0degrees Celsius under stirring conditions, gradually adding potassium permanganate under stirring and cooling, where the mass ratio of potassium permanganate and graphite powder is 3:1, and during the process, the temperature of mixture is maintained below 10degrees Celsius and continuously stirred for 10 hours, increasing the temperature of the mixture to 35degrees Celsius and again adding 98 %mass concentrated sulfuric acid and potassium permanganate to the mixture, after stirring for 24 hours, pouring the obtained mixture into frozen 10 %mass hydrogen peroxide to terminate the reaction, adding a 10 %mass hydrochloric acid solution with the same volume as the mixture and uniformly stirring, repeatedly centrifugation at 8000 rpm for 5 minutes/time and washing with distilled water until no sulfate can be detected with barium chloride solution, separating the suspension into supernatant and golden residue by centrifugation, processing at 8000 rpm for 5 minutes, and obtaining exfoliated graphene oxide in the supernatant, and (c) preparing magnetic iron(II,III) oxide/graphene oxide nanocarrier material by ultrasonically treating the graphene oxide suspension for 0.5-1.5 hours, then adding magnetic iron(II,III) oxide nanoparticles to the suspension, and further ultrasonically treating the mixture for 1 hour, and after reacting at 60degrees Celsius for 1 hour under mechanical stirring, obtaining the iron(II,III) oxide/graphene oxide composite material by magnetic separation, where the mass ratio of graphene oxide suspension and magnetic iron(II,III) oxide nanoparticles is (20-35):1.