• 专利标题:   Constructing multifunctional graphene gene vector used in e.g. gene transfection, by dispersing graphene quantum dots in water, adding branched polyethyleneimine and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and dialyzing filtrate.
  • 专利号:   CN107805642-A
  • 发明人:   CHEN Q, DONG W, LI J, SHENG W, ZUO G
  • 专利权人:   UNIV NANJING SCI TECHNOLOGY
  • 国际专利分类:   C12N015/87
  • 专利详细信息:   CN107805642-A 16 Mar 2018 C12N-015/87 201827 Pages: 8 Chinese
  • 申请详细信息:   CN107805642-A CN10816284 09 Sep 2016
  • 优先权号:   CN10816284

▎ 摘  要

NOVELTY - Method for constructing a multifunctional graphene gene vector, involves ultrasonically dispersing graphene quantum dots in water, adding 3-5 times branched polyethyleneimine with a molecular weight of 10,000-25,000, ultrasonically dispersing, adding 50-100% 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, ultrasonically dispersing, stirring overnight at room temperature, filtering the mixture, dialyzing the filtrate, and freeze-drying the filtrate. USE - The method is used for constructing multifunctional graphene gene vector which is used in gene transfection (claimed) and in cell biology research and gene therapy. ADVANTAGE - The method is simple. The constructed vector has low toxicity and transfection efficiency of 80.57%.