▎ 摘　　要

NOVELTY - Preparing ionic liquid-graphene nano composition comprises taking graphene dispersion with aqueous solution of a cationic surfactant to obtain mixture, performing an ultrasonic treatment on the mixture to obtain cationic surfactant-graphite alkene compound, mixing the cationic surfactant-graphite alkene compound with graphite diluted nano sheet to obtain surface active agent of first cation-graphite alkene compound and second cation surface active agent, and dispersing the mixture by ultrasonic disperser and ionic liquid. USE - The ionic liquid-graphite nano composition is useful for electrochemical detection (claimed). ADVANTAGE - The method provides ionic liquid-graphite nano composition with linear response range of 0.1-80 pg/ml, detection lower limit of 0.04 pg/ml, good specificity and high sensitivity. DETAILED DESCRIPTION - Preparing ionic liquid-graphene nano composition comprises taking graphene dispersion with aqueous solution of a cationic surfactant to obtain a mixture, performing an ultrasonic treatment on the mixture to obtain a cationic surfactant-graphite alkene compound, mixing the cationic surfactant-graphite alkene compound with graphite diluted nano sheet to obtain surface active agent of first cation-graphite alkene compound and second cation surface active agent, and dispersing the mixture by an ultrasonic disperser and ionic liquid, where the first cation surface active agent comprises hexadecyl trimethyl ammonium bromide, dodecyl trimethyl ammonium chloride, dodecyl trimethyl ammonium bromide, hexadecyl trimethyl ammonium chloride and quaternary ammonium salt and the second positive ion surface active agent comprises poly styrene sulfonic acid sodium, polyacrylic acid and p-methyl styrene sulfonic acid sodium. INDEPENDENT CLAIMS are included for: (1) an electrochemical immune detection method comprising: (a) obtaining ferrocene marking the antibody to be detected; (b) adding graphene dispersion with an aqueous solution of cationic surfactant, performing ultrasonic treatment on the mixture to obtain cationic surfactant-graphite alkene compound, mixing the obtained cationic surfactant-graphite alkene compound with an aqueous solution of the cationic surfactant to obtain graphene nano sheet, and dispersing the obtained sheet into an ionic liquid using the ultrasonic disperser to obtain ionic liquid-graphene nano compound; (c) taking the ferrocene marking the antibody with the ionic liquid-graphene nano compound, adding alkaline phosphatase to the obtained ionic liquid-graphene nano compound to obtain ionic liquid-graphene nano-composition-alkaline phosphatase and ferrocene mark of all antibody coupled object; (d) processing the combined antibody solution in the closed site to remove non-specifically bound object using a glass carbon electrode to obtain an electrode; (e) adding the obtained electrode with different concentration gradients of protein standard substance solution, ion liquid-graphene nano-composition alkaline phosphatase and ferrocene marking antibody to obtain a working electrode; (f) incubating the cells with electrodes to be detected in the buffer solution containing substrate ascorbyl phosphate salt, and drawing a standard curve against immune response of the current electrode and concentration of the buffer solution; and (g) incubating the electrode into the protein sample solution, performing electrochemical detection on the electrodes, and drawing a standard curve against electrochemical peak current intensity of the protein solution to obtain a concentration of the protein sample solution, where the electrodes comprises molybdenum wire electrode as counter electrode and saturated calomel electrode as a reference electrode; and (2) an electrochemical immune detection escape purine of pyrimidine de-nucleic acid incision enzyme/reduction factor, comprising obtaining ferrocene mark of the pyrimidine nucleic acid incision enzyme/reduction factor antibody from purine, taking graphene dispersion with an aqueous solution of the cationic surfactant, performing ultrasonic treatment on the obtained mixture to obtain cationic surfactant-graphite alkene compound, removing the second cation surface active agent aqueous solution from the purine to obtain graphene nano sheet, dispersing the nano sheet with the ionic liquid by the ultrasonic disperser to obtain ionic liquid-graphene nano composition, removing the ferrocene marking of nucleic acid incision enzyme/reduction factor antibody and the ionic liquid graphene nano composition from purine by mixing process, mixing alkaline phosphatase with the obtained depurine mixture to obtain ionic liquid graphene nano-composition-alkaline phosphatase and ferrocene mark of nucleic acid incision enzyme/redox cofactor B antibody coupled object, performing glass carbon electrode surface modification process on depurine of pyrimidine nucleic acid incision enzyme/redox factor antibody solution combined with closed site to remove non-specifically bound object and to obtain an electrode, taking the electrode with different concentration gradients of purine pyrimidine de-nucleic acid incision enzyme/reduction factor standard product solution, enzyme/reduction factor antibody, the ionic liquid-graphene nano-composition-alkaline phosphatase and ferrocene mark to obtain working electrode, incubating de-pyrimidine nucleic acid and purine cutting enzyme/reduction factor with electrode in buffer solution by cyclic voltammetry electrochemical detection method, drawing a standard curve against de-pyrimidine nucleic acid incision enzyme/reduction factor of the current electrode and concentration of the buffer solution, incubating the electrode into the purine pyrimidine nucleic acid incision enzyme/redox factor sample solution by using cyclic voltammetry electrochemical detection method, and drawing a standard curve against electrochemical peak current intensity of the purine pyrimidine nucleic acid incision enzyme/reduction factor of the sample solution to obtain a concentration of the purine pyrimidine nucleic acid incision enzyme.