▎ 摘　　要

NOVELTY - Ultra-sensitively detecting Mucin-1 chiral signals based on gold-graphene nano-assembly, involves coupling gold nanoparticles with Mucin-1 adapter part complementary sequence of graphene assembling to obtain metal-graphite nanometre assembly body,there is the object to be detected Mucin-1. The structure of the assembly changes, gold nanoparticles on the assembly that is the change of assembly chirality signal to realize the Mucin-1 ultra-sensitive detection. The construction of the sensor is detected. A gold nanoparticle is modified Mucin-1 aptamer Mucin-1 aptamer. USE - Method used for detecting Mucin-1 chiral signals (claimed). ADVANTAGE - The method enables to detect Mucin-1 chiral signals with circular dichroism high performance and good biological stability gold-graphite alkene self assembly nanometer structure. DETAILED DESCRIPTION - Ultra-sensitively detecting Mucin-1 chiral signals based on gold-graphene nano-assembly, involves coupling gold nanoparticles with Mucin-1 adapter part complementary sequence of graphene assembling to obtain metal-graphite nanometre assembly body,there is the object to be detected Mucin-1. The structure of the assembly changes, gold nanoparticles on the assembly that is the change of assembly chirality signal to realize the Mucin-1 ultra-sensitive detection. The construction of the sensor is detected. A gold nanoparticle is modified Mucin-1 aptamer Mucin-1 aptamer. The citric acid reduction method is used to synthesize the gold nano-particle. The obtain gold nano-particle is centrifuged and re-suspended in ultra pure water whose concentration is 5nM. 100 mu l concentrated heavy gold nano-particle suspension is collected and added to 2 mu l of 10 mu M thiolated Mucin-1 aptamer. 50 mu M of sodium chloride solution is added at 37 degrees c and incubated overnight to obtain a coupling Mucin-1aptamer of gold nano-particle solution. 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-Hydroxysuccinimide (NHS) and graphene are added in a molar ratio of 1000:1000:1 and the reaction is carried out in the darkness for 2 hours, and then resuspended in a 10 mM phosphate buffer with a pH of 7.5. Mucin-1 CS at a final concentration of 2 mu M is added for 3 hours to obtain a graphene-Mucin-1 CS solution. 100 mu l of the gold nanoparticles is used to couple the Mucin-1aptamer solution at 7500 rotations per minute for 10 minutes to remove the supernatant. 100 mu L of the graphene-Mucin-1 CS solution is filtered through an ultrafiltration tube. The last two filters are mixed in 100 mu l of 10 mM tris-borate-ethylenediaminetetraacetic acid (TBE) buffer containing 50 mM sodium chloride, 50 mM magnesium nitrate and 0.01wt.% sodium dodecyl sulfate is added and reacted for 12 hours. The standard curve of the chiral signal and Mucin-1 concentration of gold-graphene nanometer assembly is established. Rounded dichroic spectroscopy is used to establish a high-yield gold-graphene nanometer assembly. The standard curve between the two groups is established according to the relationship between the concentration of Mucin-1 and the chiral signal intensity of gold-graphene nanometer assembly, and the concentration of Mucin-1 is detected by chiral signal.