▎ 摘　　要

NOVELTY - Performing preparation method and application of a graphene/polyaniline nanowire array immunosensor comprises polishing the glassy carbon electrode having a diameter of 4 mm is sequentially with 1.0 micro m, 0.3 micro m, and 0.05 micro m alumina, and rinsing with ultrapure water. The amount of 6 microliter of palladium hybrid graphene/polyaniline solution labeled with capture antibody Ab1 at a concentration of 2-4 mg/mL is added dropwise to the surface of the glassy carbon electrode, and dried at 4 degrees C. The amount of 3 microliter of a bovine serum albumin solution with a mass fraction of 1-3 wt.% is added dropwise to block the non-specific active site on the surface of the electrode, and the surface of the electrode is rinsed with a phosphate buffer solution of pH 7.4, and dried at 4 degrees C. The amount of 6 microliter of procalcitonin standard solution or unknown concentration solution is added, incubated at 37 degrees C for 0.5-2 hours. USE - Method for performing preparation method and application of a graphene/polyaniline nanowire array immunosensor. ADVANTAGE - The method enables to perform preparation method and application of a graphene/polyaniline nanowire array immunosensor, which has simple preparation and low cost, and compensates for the defects of traditional detection technology, such as complicated operation, high cost and long time consumption. The sensor developed based on the method is applied to the actual sample detection of procalcitonin, and has high sensitivity. It has high specificity, rapid response and portability. Its detection limit is as low as 1.2fg/mL, and the linear range is as wide as 5fg/mL-50ng/mL. It has great potential application in the early clinical detection of procalcitonin. DETAILED DESCRIPTION - Performing preparation method and application of a graphene/polyaniline nanowire array immunosensor comprises polishing the glassy carbon electrode having a diameter of 4 mm is sequentially with 1.0 micro m, 0.3 micro m, and 0.05 micro m alumina, and rinsing with ultrapure water. The amount of 6 microliter of palladium hybrid graphene/polyaniline solution labeled with capture antibody Ab1 at a concentration of 2-4 mg/mL is added dropwise to the surface of the glassy carbon electrode, and dried at 4 degrees C. The amount of 3 microliter of a bovine serum albumin solution with a mass fraction of 1-3 wt.% is added dropwise to block the non-specific active site on the surface of the electrode, and the surface of the electrode is rinsed with a phosphate buffer solution of pH 7.4, and dried at 4 degrees C. The amount of 6 microliter of procalcitonin standard solution or unknown concentration solution is added, incubated at 37 degrees C for 0.5-2 hours, the electrode surface is rinsed with phosphate buffer solution of pH 7.4, and dried at 4 degrees C. The amount of 6 microliter of a concentration of 2-4 mg/mL of the detection antibody Ab2 labeled cerium-doped tin disulfide-luminol solution is added dropwise. The surface of the electrode is rinsed with a phosphate buffer solution of pH 7.4, dried at 4 degrees C, and the sensor is constructed.