▎ 摘 要
NOVELTY - Nanoenzyme cascade bioreactor is a compound obtained by connecting a substance A to the surface of a substance B. The substance A is carbon dot nanoenzyme coupled with hypoxic inhibitor. The substance B is an exosome. USE - Nanoenzyme cascade bioreactor used medicament for treatment of tumor. ADVANTAGE - The nanoenzyme cascade bioreactor has carbon dot nanoenzyme, which is modified by utilizing exosome in a bionic manner, so that the enrichment capacity of tumor tissues is improved, natural NADPH oxidase 2 on the surface of the exosome is used for providing more catalytic substrates for the carbon dot nanoenzyme, efaproxiral (RSR13) is used to improve tumor tissue hypoxia, providing more oxygen for NADPH oxidase 2, and realize the synergistic linkage and synergism to inhibit the growth of the tumor. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: Method of preparing nanoenzyme cascade bioreactor, which involves: (1) coupling a hypoxic inhibitor on the surface of the carbon-point nano enzyme to obtain carbon-point nano enzyme coupled with the hypoxic inhibitor; (2) connecting a CD63 antibody to the free carboxyl on the surface of the carbon-point nanoenzyme coupled with the hypoxic inhibitor to obtain the carbon-point nanoenzyme coupled with the CD63 antibody and the hypoxic inhibitor; and (3) connecting the carbon dot nanoenzyme coupled with the CD63 antibody and the hypoxic inhibitor on the surface of the exosome to obtain the nanoenzyme cascade bioreactor. Medicament for treating tumor, which comprises the nanoenzyme cascade bioreactor.