▎ 摘　　要

NOVELTY - Detecting an infection in a subject's ear, comprises (a) obtaining a sample of cerumen, the cerumen being adsorbed onto an applicator, (b) extracting the cerumen from the applicator, the extraction comprises contacting the applicator with a solvent, (c) measuring a presence, absence or amount of a compound in the cerumen, optionally where the compound is redox-active and associated with the infection, the measuring comprises contacting the compound with an electrochemical sensor comprises a working electrode and a reference electrode, and electrochemically measuring a current flow, which is correlated with the presence, absence or amount of the compound. USE - The method is useful for detecting an infection in a subject's ear, where the infection is Pseudomonas aeruginosa infection, otitis externa, otitis media, and otitis interna, and the subject is non-human animal i.e. captive animal, pet animal, a farm animal, or a zoo animal or a canine (all claimed). ADVANTAGE - The method allows efficient extraction of cerumen sample and require lesser amount of cerumen fluid extract. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a kit for detecting an infection in a subject's ear, comprising, (aa) an applicator for adsorbing a sample of cerumen, (bb) a solvent for extracting the cerumen and/or a compound within the cerumen from the applicator, and, optionally (cc) an electrochemical sensor, comprising a working electrode and a reference electrode and being suitable for electrochemically measuring a current flow through the sensor, which is correlated with the presence, absence or amount of the compound.