▎ 摘　　要

NOVELTY - Preparation of multifunctional magnetic targeting nano-medicine carrier involves dissolving bimetallic particles in deionized water, ultrasonically processing, adding molybdate, acid solution and polyvinylpyrrolidone, heating, washing, vacuum drying to obtain silver and carbon-doped ferroferric oxide nanoparticles, ultrasonically dispersing nanoparticles, heating in oil bath to 40degrees Celsius, injecting ethanol, ammonia and surfactant into the mixed solution, stirring at room temperature for 30 minutes, adding 125 muL tetraethyl orthosilicate, stirring, magnetically separating the product, alternately washing product with ethanol and water, reacting at 100degrees Celsius for 2 hours, washing, adding bio-cellulase and other biomass protease, and mixing with the obtained complex to form protective shell with bio-enzyme, etching away inner silicon dioxide layer with sodium hydroxide solution to form cavity structure, mixing with graphene oxide solution, hydrothermally reacting, washing, and drying. USE - Preparation of multifunctional magnetic targeting nano-medicine carrier (claimed) for biomolecular imaging process and photothermal therapy. ADVANTAGE - The preparation method provides nano-medicine carrier with strong magnetic field response mechanism and cavity structure which improves targeted drug delivery efficiency and forms biological enzyme protection outer layer to improves the biocompatibility of the carrier and reduces the problem of systemic drug release. DETAILED DESCRIPTION - Preparation of multifunctional magnetic targeting nano-medicine carrier involves dissolving iron salt and biomass in deionized water, dripping aqueous ammonia to adjust the pH to more than 10, adding to high-pressure reactor, hydrothermally reacting at 200degrees Celsius for 10 hours, cooling, vacuum filtering to obtain red color product, washing with deionized water, drying at 60degrees Celsius in constant temperature drying oven for 12 hours, and adding to clean crucible and calcining at 450degrees Celsius for 2 hours to obtain carbon-doped ferroferric oxide nanoparticles, dissolving soluble silver salt in organic solvent, mixing, heating to 70degrees Celsius, adding dispersed ferroferric oxide solution, reacting at 90degrees Celsius for 2 hours, cooling to room temperature, magnetically separating the product and repeatedly cleaning with ethanol to obtain bimetallic nanoparticles, dissolving 100 mg bimetallic particles in 50 mL deionized water, ultrasonically processing for 20 minutes, adding molybdate, acid solution and polyvinylpyrrolidone, heating to 240degrees Celsius for 12 hours, washing several times with ultrapure water and absolute ethanol, magnetically separating, vacuum drying in oven at 60degrees Celsius to obtain silver and carbon-doped ferroferric oxide nanoparticles, ultrasonically dispersing the silver and carbon-doped ferroferric oxide nanoparticles for 20 minutes, heating in oil bath to 40degrees Celsius while mechanically stirring, injecting ethanol, ammonia and surfactant into the mixed solution, stirring at room temperature for 30 minutes, adding 125 muL tetraethyl orthosilicate, stirring for 6 hours, magnetically separating the product, alternately washing product with ethanol and water, finally reacting in reactor at 100degrees Celsius for 2 hours, washing with deionized water, adding bio-cellulase and other biomass protease, and mixing with the obtained complex to form protective shell with bio-enzyme, etching away inner silicon dioxide layer with sodium hydroxide solution to form cavity structure, mixing with graphene oxide solution, hydrothermally reacting at 200degrees Celsius, washing with ultrapure water and absolute ethanol for multiple time, magnetically separating, and vacuum drying in oven at 60degrees Celsius. The biomass protease is one of biocellulase, amylase, and saccharification enzyme. An INDEPENDENT CLAIM is included for the multifunctional magnetic targeting nanomedicine carrier, which is prepared by above preparation process. The multifunctional magnetic targeting nanomedicine carrier is silver and carbon-doped ferroferric oxide/manganese oxide/reduced graphene oxide (Fe3O4-Ag:C:MnO2/rGO) nano-medicine carrier, and has magnetic saturation of 105 emu/g, saturation loading rate of 87%, drug release rate of 70.3% under the acidic buffer pH condition of 4.1, and biocompatibility of 78.2%.