▎ 摘　　要

NOVELTY - Preparation of gold/graphene /titanium dioxide nanotubes array/titanium sensing electrode involves (s1) mixing 34-36 volume parts glycerol and 4.0-6.0 volume parts ultrapure water, adding 0.39-0.41 parts mass ammonium fluoride crystals to obtain electrolyte (A), using titanium sheet as positive electrode and platinum sheet as negative electrode, inserting positive electrode and negative electrode in electrolyte solution (A) to obtain titanium dioxide nanotubes array/titanium sheet, (s2) placing in chamber, introducing hydrogen and inert gas in chamber, raising heating to obtain the graphene/titanium dioxide nanotubes array/titanium sheet, and (s3) placing counter electrode, reference electrode and graphene/titanium dioxide nanotubes array/titanium sheet in electrolyte (B) as working electrode, and using multi-potential step method. The electrolyte (B) is mixture of tetrachloroauric acid and water. USE - Preparation of gold/graphene /titanium dioxide nanotubes array/titanium sensing electrode in detecting biomarkers concentration (all claimed). ADVANTAGE - The preparation method has simple process and convenient operation. The electrode can be modified by electrochemical method and detection of the sample to be tested can be realized, the stability is good, the detection limit is low, and the test sensitivity and accuracy are high, inexpensive, and is good for civil use. DETAILED DESCRIPTION - Preparation of gold/graphene /titanium dioxide nanotubes array/titanium sensing electrode involves (s1) mixing 34-36 volume parts glycerol and 4.0-6.0 volume parts ultrapure water, adding 0.39-0.41 parts mass ammonium fluoride crystals to obtain electrolyte (A), turn on direct current stabilized power supply, using titanium sheet as positive electrode and platinum sheet as negative electrode, inserting positive electrode and negative electrode in electrolyte solution (A), and anodizing for 1-2 hours under the condition of stirring the electrolyte solution (A) to obtain titanium dioxide nanotubes array/titanium sheet, (s2) placing the titanium dioxide nanotubes array/titanium sheet in chamber of chemical vapor deposition device, introducing hydrogen and inert gas in chamber, raising heating to 850-1050℃, introducing methane to chamber for 1-6 minutes to obtain the graphene/titanium dioxide nanotubes array/titanium sheet, and (s3) placing counter electrode, reference electrode and graphene/titanium dioxide nanotubes array/titanium sheet in electrolyte (B) as working electrode, using multi-potential step method on graphene/titanium dioxide nanotubes array/titanium sheet surface electrochemical reduction deposition gold nanoparticle 90-180 seconds. The electrolyte (B) is mixture of tetrachloroauric acid and water. An INDEPENDENT CLAIM is included for use of gold/graphene /titanium dioxide nanotubes array/titanium sensing electrode in detecting biomarkers concentration.