▎ 摘　　要

NOVELTY - Manufacturing graphene-metal and quantum dot-porous silica composite nano-material sheet core electrochemical luminous immune sensor comprises providing the chromatographic paper with wax printer channel pattern, heating wax printing of paper with electronic temperature controlling apparatus, roasting wax and evenly penetrating into the chromatographic paper to print the wax to form a hydrophilic channel, selecting the clinical incidence higher tumor marker for measuring, and preparing the graphene-metal composite nano material using a material. USE - The method is useful for manufacturing graphene-metal and quantum dot-porous silica composite nano-material sheet core electrochemical luminous immune sensor (claimed). ADVANTAGE - The method economically provides graphene-metal and quantum dot-porous silica composite nano-material sheet core electrochemical luminous immune sensor with high sensitivity and strong specificity. DETAILED DESCRIPTION - Manufacturing graphene-metal and quantum dot-porous silica composite nano-material sheet core electrochemical luminous immune sensor comprises (1) providing the chromatographic paper with wax printer channel pattern, heating wax printing of paper with electronic temperature controlling apparatus, roasting wax and evenly penetrating into the chromatographic paper to print the wax to form a hydrophilic channel, (2) selecting the clinical incidence higher tumor marker for measuring, (3) preparing the graphene-metal composite nano material using a material, (4) preparing the quantum dot-porous silica composite nanomaterial and marking secondary antibody, (5) putting the graphite alkene-metal composite nano-material into paper core after the channel chip to improve conductivity and sensitivity of paper core, (6) modifying obtained step (5) for capturing antibody of thionine, (7) modifying the quantum dot-porous silicon composite material of anti-modification step (6) of paper on the surface of the chip using antigen antibody specific recognition and surface modification technology, and making electrochemical luminescence sensor, (8) combining good processing the paper core with silk screen printing electrode to detect electrochemical lighting signal, (9) polishing powder using silk screen printing electrode and graphite alkene-metal composite nanomaterial including graphene-copper, graphene-cobalt, graphene-platinum, where the quantum dot-porous silicon material is a cadmium-tellurium quantum dot-porous silicon nanometre material comprising printing the experiment film electrode using aluminum oxide polishing powder for polishing electrode, and wax printer, and printing wax to chromatography paper using electronic temperature control instrument and then heating to make the wax uniformly penetrates into the chromatographic paper, without print wax formed paper core channel, preparing the graphene-metal and the quantum dotporous silica composite nano material and then adding 100-200 ml of graphenemetal solution to drop a region as a channel region, completely drying the graphene-metal solution on the paper and a region and then adding 10-100 mu l of thionine solution, combining the thionine solution on the paper with carbon nano-tube and completely absorbing on the paper and a region and then adding 10-100 mu l of glutaric dialdehyde solution and naturally drying, washing obtained product by super-pure water for three times, where washing method comprises front dropping buffer solution, the reverse face with water absorbent paper, water permeable and a region adding 1-10 mu l of the first resistance, repeating washing, where washing is not adsorbed or absorbed is not firm and the antibody to be an anti-solution is a region absorbing firmly and then dripping 20-100 mu l of bovine serum albumin solution to seal the can to obtain active site, repeating washing, adding 1-10 mu l of antigen into a region and then completely reacting, when the reaction is finished, repeating washing, eluting the buffer solution in a region corresponding to the dripping 5-20 mu l of porous silicon quantum dot-marked secondary antibody to make it fully react, placing the paper core passage in the prepared film electrode and then dropping buffer solution with pH value to 7.4 and then combining with electrochemical luminescence instrument to measure, that is capable of accurately determining the concentration of antigen in the sample.