▎ 摘　　要

NOVELTY - Culture medium is based on Murashige and Skoog (MS) medium, and includes 1.5-2.5mg/L graphene, 0.8-1.5mg/L 6-Benzylaminopurine (6-BA), 0.7-1.2mg/L Indole-3-butyric acid (IBA). The pH value of the basic medium is 5.8-6.2, and the pH value of the medium for promoting the stable differentiation of raspberry callus is 5.8-6.2. USE - Culture medium for promoting stable differentiation of Raspberry callus and for raspberry tissue culture expansion (claimed). ADVANTAGE - The culture medium has graphene, which effectively improves the stable differentiation efficiency of raspberries in tissue culture and promote plant growth, such as penetrating the seed epidermis, promotes the increase of seed water content and germination, promotes the expression of aquaporin gene in plant roots, promotes root growth and graphene is used to play a bactericidal role in the culture medium. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a raspberry tissue culture propagation method, which involves inoculating sterilized raspberry explant in the medium promoting the stable differentiation of raspberry callus, inducing differentiation culture under budding conditions to obtain adventitious buds, inoculating adventitious shoots in 6-BA MS medium, inducing differentiation culture under rooting conditions to obtain adventitious roots.