• 专利标题:   Graphene oxide-lysozyme/basic fibroblast growth factor composite dressing preparation involves purchasing graphene oxide film, dissolving by adding tris hydrochloric acid in deionized water, and then adding dopamine powder.
  • 专利号:   CN111744051-A
  • 发明人:   ZHOU D, LI D, ZHANG T, PENG J, LI H
  • 专利权人:   GEN HOSPITAL WESTERN THEATER COMMAND
  • 国际专利分类:   A61L015/18, A61L015/26, A61L015/42, A61L015/44, A61L015/46
  • 专利详细信息:   CN111744051-A 09 Oct 2020 A61L-015/26 202088 Pages: 14 Chinese
  • 申请详细信息:   CN111744051-A CN10681521 15 Jul 2020
  • 优先权号:   CN10681521

▎ 摘  要

NOVELTY - Graphene oxide-lysozyme/basic fibroblast growth factor composite dressing preparation involves purchasing graphene oxide film, dissolving by adding 131.14 g tris hydrochloric acid in 100 ml deionized water, and then adding 200 mg dopamine powder to the obtained tris solution to obtain tris-dopamine solution with a concentration of 2 mg/ml and a pH of 8.5. The graphene oxide film is soaked in the prepared solution for 12 hours, transferred the mixture to a vibrating screen at 37 degrees C. The daptomycin powder is dissolved in deionized water to prepare a 10 mg/mL alkaline fibroblast growth factor aqueous solution, carefully washed with the graphene oxide sample and deionized water, and then incubated in daptomycin at 37 degrees C for 12 hours. The prepared graphene oxide-dopamine membrane is dissolved in basic fibroblast growth factor solution at 37 degrees C for 12 hours. USE - Method for preparing graphene oxide-lysozyme/basic fibroblast growth factor composite dressing. ADVANTAGE - The method prepares the graphene oxide-lysozyme/basic fibroblast growth factor composite dressing in a simple, cost-effective and eco-friendly manner with better antibacterial effect and growth-promoting effect, and great benefits for wound healing. DETAILED DESCRIPTION - Graphene oxide-lysozyme/basic fibroblast growth factor composite dressing preparation involves purchasing graphene oxide film, dissolving by adding 131.14 g tris hydrochloric acid in 100 ml deionized water, and then adding 200 mg dopamine powder to the obtained tris solution to obtain tris-dopamine solution with a concentration of 2 mg/ml and a pH of 8.5. The graphene oxide film is soaked in the prepared solution for 12 hours, transferred the mixture to a vibrating screen at 37 degrees C. The daptomycin powder is dissolved in deionized water to prepare a 10 mg/mL alkaline fibroblast growth factor aqueous solution, carefully washed with the graphene oxide sample and deionized water, and then incubated in daptomycin at 37 degrees C for 12 hours. The prepared graphene oxide-dopamine membrane is dissolved in basic fibroblast growth factor solution at 37 degrees C for 12 hours. The graphene oxide membrane-dopamine is added to the same concentration of daptomycin and epidermal growth factor mixture, incubated at 37 degrees C for 12 hours to prepare the graphene oxide-lysozyme/basic fibroblast growth factor.