▎ 摘　　要

NOVELTY - Electrochemical biosensor (10) device comprises two electrodes, each comprised of an electrically conductive material; one layer of graphene, graphene oxide, reduced graphene oxide or other graphene derivative, the one graphene layer (12) having incorporated there or deposited there one non-enzymatic catalytic agent to provide a non-enzymatic catalytic graphene layer for promoting a non-enzymatic oxidation-reduction reaction of the target analyte (22); and a substrate for supporting one of the electrodes and portion of the one non-enzymatic catalytic graphene layer; the device further comprises one semi-permeable membrane formed over a portion of the non-enzymatic catalytic graphene layer, the membrane being configured to allow selective movement of the target analyte from the biological fluid to the catalytic graphene layer. USE - Electrochemical biosensor device for detecting one target analyte in a biological fluid, where the target analyte is a metabolite or a hormone, preferably selected from glucose, lactate and cortisol (claimed). ADVANTAGE - The electrochemical biosensor device is that materials are layered in the device architecture in such a way that no harmful or potentially harmful material gets in contact with the skin surface and therefore no skin irritation is produced by wearing the device in deep contact with skin, even for an extended period of time, and the method enables data extracted from the humidity sensor allows the establishment of a baseline where the measurement can be taken reliably from the graphene -based sensor related to the species of interest, and data extracted from the temperature sensor may be used to compensate the drift of the sensor response due to the reaction kinetics in order to improve the accuracy of the sensor output in relation to the actual concentration of the species of interest in sweat. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a non-invasive method for detecting target analytes in biological fluid, which involves absorbing biological fluid onto the substrate of an electrochemical sensor; applying a voltage to the two or more electrodes to provide a potential difference between the two electrodes; and measuring electric current flowing between the electrodes to provide an amperometric reading generated by excess electrons produced by the oxidation-reduction reaction of the target analyte to determine at least a presence of the target analyte in the biological fluid; and (2) a method for assembling an electrochemical sensor, which involves depositing or incorporating one non-enzymatic catalytic agent on or in one layer of graphene, graphene oxide, reduced graphene oxide or other graphene derivative layer to provide one non-enzymatic catalytic graphene layer, the catalytic agent promoting an oxidation-reduction reaction of a target analyte for the sensor; assembling two electrodes and the one non-enzymatic catalytic graphene layer onto a substrate; and depositing a semi-permeable membrane over portion of the non-enzymatic catalytic graphene layer. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of an electrochemical biosensor device. 10Electrochemical biosensor 12Graphene layer 13Catalytic particle 22Target analyte 40Substrate