▎ 摘　　要

NOVELTY - Measuring fatty acid in black fungus involves weighing the sample to be tested in the test tube, homogenizing to obtain the sample slurry, centrifuging the sample slurry to obtain supernatant and residue A, adding marking agent A and purifying agent B in the residue A, stirring uniformly, filtering to obtain the extracting liquid, mixing with the supernatant to obtain the sample solution to be hydolyzed, hydrolyzing the sample liquid by 13C isotope-labelled methanol-derived reaction, hydrolyzing and esterifying under the water bath condition, cooling, drying the obtained crude extract by nitrogen blowing, re-dissolving the crude extract by normal hexane to obtain normal hexane phase, and concentrating the normal hexane phase by nitrogen blowing to obtain the upper machine analysis liquid. USE - Method for measuring fatty acid in black fungus. ADVANTAGE - The method enables measuring fatty acid in black fungus with better effect of purifying the black fungus substrate and obtaining the content of the fatty acid by further detecting whether the impurity with interference to the detection result in the black fungus is removed.