▎ 摘　　要

NOVELTY - Method (900) for electrically differentiating bases or identifying biomolecules (920) involves configuring reservoir (910) separated into two parts by a membrane (905) having nanopore connected to the two parts of reservoir; filling nanopore and reservoir with ionic buffer; where membrane comprises graphene layer and insulating layers (915); forming graphene transistor in which current is modulated by charges/dipoles passing through nanopore, and corresponding to nucleobase and biomolecule; and where nanopore is coated with organic layer to interact with nucleobase and biomolecule. USE - For identifying and differentiating molecules using a graphene nanopore transistor useful for DNA sequencing and biomolecular sensing. ADVANTAGE - The field effect transistors (graphene transistor) for sensing biomolecules, are suitable for reducing the required amount of reagents by leveraging their high sensitivity. Single molecules can be driven through the nanopore one by one, and as they go through, each individual molecule can modulate the current through the graphene transistor; this configuration allows molecular detection with single molecule accuracy. As the graphene layer can be as thin as 0.335 nm, which is enough for DNA sequencing purpose. DETAILED DESCRIPTION - Method (900) for electrically differentiating bases or identifying biomolecules (920) involves configuring a reservoir (910) separated into two parts by a membrane (905); configuring a nanopore formed through the membrane, the nanopore connecting the two parts of reservoir; filling the nanopore and reservoir with ionic buffer; where membrane comprises graphene layer and insulating layers (915); where graphene layer is wired to first and second metal pads to form graphene transistor in which transistor current flowing through graphene transistor is modulated by charges or dipoles passing through nanopore, the charges or dipoles corresponding to a nucleobase and biomolecule; and where nanopore is coated with organic layer configured to interact with nucleobase differently than other nucleobases and interact with biomolecule differently than other biomolecules (925). DESCRIPTION OF DRAWING(S) - The figure shows flow chart of method for differentiating and identifying bases and biomolecules in a nanopore. method for electrically differentiating bases and/or identifying biomolecules (900) Membrane (905) Reservoir (910) Insulating layers (915) Biomolecule (920) Other biomolecules (925)