• 专利标题:   Method for immobilizing bovine serum albumin, involves combining bovine serum albumin with surface of poly-dopamine/oxidized graphene film under action of hydrogen bond and hydrophobicity.
  • 专利号:   CN103357452-A, CN103357452-B
  • 发明人:   LIANG R, LIU C, QIU J
  • 专利权人:   UNIV NANCHANG
  • 国际专利分类:   B01D015/08, B01L003/00
  • 专利详细信息:   CN103357452-A 23 Oct 2013 B01L-003/00 201421 Pages: 9 Chinese
  • 申请详细信息:   CN103357452-A CN10267612 29 Jun 2013
  • 优先权号:   CN10267612

▎ 摘  要

NOVELTY - A bovine serum albumin immobilization method involves pumping dopamine and oxidized graphene mixed solution into a micro-fluidic chip separation channel by using a vacuum pump such that the dopamine fixes the oxidized graphene to a surface of the separation channel to form a poly-dopamine/oxidized graphene film in a self-polymerization manner. The bovine serum albumin is combined with the surface of graphene film under the action of hydrogen bond and hydrophobicity to obtain a poly-dopamine/oxidized graphene/ bovine serum albumin functionalized micro-fluidic chip channel. USE - Method for immobilizing bovine serum albumin. ADVANTAGE - The method enables enhancing loading capacity of bovine serum albumin in the micro-fluidic chip separation channel, maintaining biological activity of the bovine serum albumin and providing universal platform for high-efficiency separation of an amino acid antipode and a dipeptide antipode. The bovine serum albumin immobilized using the method has high stability, biocompatibility and hydrophilicity.