▎ 摘　　要

NOVELTY - Detecting ochratoxin A in beer based on core-shell type upconversion luminescent nanomaterial and oxidized graphene luminescence resonance energy transfer, is claimed. Luminous intensity of the core-shell type upconversion luminescent sodium yttrium fluoride-ytterbium-erbium nanomaterials (I) is 2.74 times the bare nucleus upconversion luminescent sodium yttrium fluoride-ytterbium-erbium nanomaterials (II). Ochratoxin A aptamers are coupled with (I) to form core-shell type energy donor probes, and luminescent spectra of (I) and absorption spectra of oxidized graphene can be overlapped. USE - The method is useful for detecting ochratoxin A in beer based on core-shell type upconversion luminescent nanomaterial and oxidized graphene luminescence resonance energy transfer (claimed). ADVANTAGE - The method: has high detection sensitivity and accurate results; and is fast and convenient, and suitable for beer sample detection. DETAILED DESCRIPTION - Detecting ochratoxin A in beer based on core-shell type upconversion luminescent nanomaterial and oxidized graphene luminescence resonance energy transfer, is claimed. Luminous intensity of the core-shell type upconversion luminescent sodium yttrium fluoride-ytterbium-erbium nanomaterials of formula (NaYF4:Yb(0.18), Er 0.02/NaYF4) (I) is 2.74 times the bare nucleus upconversion luminescent sodium yttrium fluoride-ytterbium-erbium nanomaterials of formula (NaYF4:Yb(0.18), Er(0.02)) (II). Ochratoxin A (OTA) aptamers are coupled with (I) to form core-shell type energy donor probes, and luminescent spectra of (I) and absorption spectra of oxidized graphene (GO) can be effectively overlapped, where oxidized graphene is used as energy receptor probe. The core-shell type energy donor probe is first incubated with OTA, and then the aptamer preferentially binds to OTA. The core-shell type energy donor probe (which is bound to OTA) is not adsorbed to the GO surface when GO is added. The core-shell-type energy donor probe (which is not bound to OTA), is partially luminescent at 541 nm and concentration of 0.001-250 ng/ml, where OTA concentration and 541 nm upconversion luminescence signal is positively correlated, and a standard curve is established to achieve quantitative detection of OTA.