▎ 摘 要
NOVELTY - An algal toxin degrading enzyme comprising (a) an algal toxin degrading enzyme comprising a 738 amino acid sequence (SEQ ID NO: 2) fully defined in the specification, a 738 amino acid sequence (SEQ ID NO: 3) fully defined in the specification, or a 738 amino acid sequence (SEQ ID NO: 4) fully defined in the specification, or (b) a protein derived from the enzyme (a) in which the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4 has been substituted, deleted, or added with one or more amino acids, and the enzyme activity is unchanged, or a protein comprising an amino acid sequence with a tag attached to the amino terminal and/or carboxy terminal of SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4, is new. USE - The algal degrading enzyme is used for inhibiting the growth of cyanobacteria and/or degrading algal toxins (claimed). ADVANTAGE - The production of the modified algal toxin degrading enzyme and the activity of the enzyme to degrade the algal toxin are higher than the wild-type algal toxin degrading enzyme before the modification, and both the output and the enzyme activity are improved, and the half-life at 0 degrees C is also longer. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a gene capable of encoding the algal toxin degrading enzyme; (2) an expression vector comprising the gene; (3) an expression strain comprising the expression vector; (4) preparing and expressing algal toxin degrading enzyme, comprising (1) culturing the expression strain to induce the expression of genes encoding algal toxin degrading enzymes, and (2) separating and purifying the expressed algal toxin degrading enzyme; and (5) a composite material with a function of degrading algal toxins, comprising a graphene material and the algae toxin degrading enzyme of claim 1 immobilized on the graphene material, where the preparation of the composite material, comprises mixing graphite powder, acid, oxidant and additives in an ice water bath, heating the mixture at 35-45 degrees C, and stirring for 10-60 minutes, continuously heating at 90-100 degrees C and stirring for 10-20 minutes, mixing with hydrogen peroxide aqueous solution after cooling to room temperature, separating solid and liquid, washing and drying the solid phase.