▎ 摘 要
NOVELTY - Preparing reduced graphene oxide/bioglass nanofiber scaffold involves: (1) weighing granular bacterial cellulose block, putting into a cell wall breaking machine, adding a water and beating into paste to obtain a bacterial cellulose pulp-like solution; (2) ultrasonically treating graphene oxide to obtain uniformly dispersed graphene oxide solution, heating, dripping hydrazine hydrate solution into the mixture, reacting and then centrifuging to obtain the reduced graphene oxide solution; and (3) uniformly mixing the bacterial cellulose solution and the reduced graphene oxide solution to obtain a reduced graphene oxide/bacterial cellulose mixed solution, adding calcium-, silicon- and phosphorus-source to the mixed solution, stirring, leaving still to obtain a biological glass gel of the mixture, and freeze-drying to obtain a reduced graphene oxide/bacterial cellulose/biological glass precursor fiber scaffold; and (4) calcining to obtain the product. USE - The method is useful for preparing reduced graphene oxide/bioglass nanofiber scaffold, which is useful in bone transplantation and bone replacement. ADVANTAGE - The method enables simple, rapid and economical preparation of the reduced graphene oxide/bioglass nanofiber scaffold with high antibacterial property and biological activity. DETAILED DESCRIPTION - Preparing reduced graphene oxide/bioglass nanofiber scaffold involves: (1) weighing a certain amount of granular bacterial cellulose block, putting into a cell wall breaking machine, adding a certain amount of water and beating into paste to obtain a bacterial cellulose pulp-like solution; (2) putting graphene oxide into an ultrasonic machine to carry out ultrasonic treatment for 1 hour to obtain uniformly dispersed graphene oxide solution, heating at 100 degrees C, dripping hydrazine hydrate solution into the mixture, reacting for 1 hour, and then centrifuging to obtain the reduced graphene oxide solution, where the mass ratio of graphene oxide and hydrazine hydrate solution is 1:1000; and (3) uniformly mixing the bacterial cellulose pulp-like solution obtained in step (1) and the reduced graphene oxide solution obtained in step (2) in a volume ratio of 10:1 to obtain a reduced graphene oxide/bacterial cellulose mixed solution, adding calcium source, silicon source and phosphorus source, preferably calcium nitrate tetrahydrate, tetraethyl orthosilicate and triethyl phosphate to the reduced graphene oxide/bacterial cellulose mixed solution, stirring for 1 hour, leaving still for 3-10 hours to obtain a biological glass gel of the mixture, and freeze-drying to obtain a reduced graphene oxide/bacterial cellulose/biological glass precursor fiber scaffold; and (4) calcining the obtained precursor fiber scaffold in the presence of argon atmosphere at 550-800 degrees C for 1-6 hours to obtain the product.