▎ 摘　　要

NOVELTY - Controlling the speed of a polypeptide passing through a nanopore, comprises: (i) linking a polypeptide with a nucleic acid to obtain a nucleic acid-polypeptide linker; (ii) applying a voltage across the nanopore in the presence of a nuclease and controlling the speed of the linked polypeptide passing through the nanopore. The nucleic acid-polypeptide linker is passed through the nanopore, and the nuclease controls the movement of the nucleic acid. USE - The method is useful for controlling the speed of a polypeptide passing through a nanopore. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) measuring the electrical signal waveform of a polypeptide, comprising: (i) connecting the polypeptide to the nucleic acid to obtain a nucleic acid-polypeptide linker, (ii) in the presence of nuclease, apply a voltage across the nanopore to allow the nucleic acid-polypeptide linker to pass through the nanopore, and control the movement of the nucleic acid through the nuclease, where controlling the speed of the linked polypeptide passing through the nanopore, (iii) reading the nanopore current signal to obtain the electrical signal waveform of the polypeptide; (2) determining the amino acid sequence of a polypeptide comprising performing sequence determination by measuring the electrical signal generated by the polypeptide through a nanopore sensor; and (3) identifying a polypeptide or its part by measuring the electrical signal generated by the polypeptide through a nanopore sensor for identification.