▎ 摘　　要

NOVELTY - Method for preparing hypericin nanocomplex material, involves mixing graphene oxide dispersion and hydrofluoric acid, performing sonication of the mixture, transferring the mixed solution to polytetrafluoroethylene-lined autoclave mixture, naturally cooling the polytetrafluoroethylene-lined autoclave mixture, filtering the mixture, performing centrifugal filtration, freeze-drying, adding water, performing sonication, adding fluorinated graphite to obtain dispersion, dialyzing the fluorinated graphene aqueous solution, freeze-drying the purified fluorinated graphene, adding sodium hydroxide and sodium hypochlorite, placing the mixture, converting hydroxyl group on layer to carboxyl group, neutralizing, centrifuging, freeze-drying, drying the mixture, dispersing the mixture in water, adding hypericin, 1-ethyl-(3-dimethyl aminopropyl)carbodiimide hydrochloride and 4-dimethylaminopyridine, filtering the mixture, and subjecting the mixture to freeze-drying operation. USE - The method is useful for preparing hypericin nanocomplex material (claimed) and treating cancer cells. ADVANTAGE - The method increases stability of polymer nanomaterial. DETAILED DESCRIPTION - Method for preparing hypericin nanocomplex material using hyaluronic acid modified fluorinated graphene as carrier, involves mixing 1-3 mg/ml 40-60 ml graphene oxide dispersion and 35-45% 0.5-0.75 ml hydrofluoric acid, performing sonication of the mixture for 1-2 minutes to obtain mixed solution, transferring the mixed solution to polytetrafluoroethylene-lined autoclave mixture, maintaining the mixture at 180-200 degrees C for 30-40 hours, naturally cooling the polytetrafluoroethylene-lined autoclave mixture to room temperature, filtering the mixture using microporous membrane, after repeated washing, performing centrifugal filtration to obtain solution, freeze-drying to obtain fluorinated graphene, obtaining fluorinated graphene, adding water, performing sonication of the mixture under ice-bath conditions for 1-2 hours, adding fluorinated graphite to obtain dispersion, removing the precipitate by centrifugation to obtain fluorinated graphene aqueous solution, dialyzing the fluorinated graphene aqueous solution for 2-3 days to obtain purified fluorinated graphene dispersion solution, freeze-drying the purified fluorinated graphene, dispersing 100-150 mg fluorinated graphene, dispersing the mixture in 80-120 ml deionized water, adding 5-7 g sodium hydroxide and 5-7 g sodium hypochlorite, placing the mixture in ultrasonic bath for 2-3 hours, converting hydroxyl group on the layer to carboxyl group, after the reaction is completed, neutralizing with diluted hydrochloric acid, centrifuging, collecting solution of the upper layer, dialyzing with water for 40-50 hours to remove unreacted water-soluble substances, adding 100-150 mg fluorinated graphene, dispersing the mixture in 25-50 ml dimethyl sulfoxide (DMSO), adding 50-75 ml 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to activate stirring reaction for 3-5 hours to obtain activated fluorinated graphene solution, weighing 175-200 mg polyethylenimine (PEI), dissolving the mixture in 75-125 ml DMSO under stirring, adding obtained PEI solution to the activated fluorinated graphene solution, ultrasonically stirring the mixture at room temperature for 24-36 hours, removing unreacted impurities by dialysis, freeze-drying to obtain fluorinated graphene-PEI, dissolving 0.5-0.6 g hyaluronic acid in 25-50 ml water, adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide to activate reaction for 3-5 hours to obtain an activated hyaluronic acid solution, weighing 60-90 mg fluorinated graphene-PEI, dissolving the mixture in 15-45 ml water, adding fluorinated graphene-PEI solution to activated hyaluronic acid solution, stirring the mixture at room temperature for 24-36 hours, performing dialysis treatment with sodium bicarbonate solution, changing water for every 3-5 hours, performing dialysis after 40-50 hours, removing unreacted impurities, removing the water in solution by rotary evaporation method, washing with acetone, drying the mixture at vacuum at 40-50 degrees C to obtain hyaluronic acid-modified fluorinated graphene, obtaining 64.8-66.8 mg hyaluronic acid modified fluorinated graphene, dispersing the mixture in 40-60 ml water, adding 6.05-6.25 mg hypericin, 2.76-2.96 mg 1-ethyl-(3-dimethyl aminopropyl)carbodiimide hydrochloride and 0.30-0.32 mg 4-dimethylaminopyridine, stirring the mixture, filtering the mixture using ultrafiltration tube, and subjecting the mixture to freeze-drying operation to obtain finished product.