• 专利标题:   Graphene aerogel useful in enrichment of food-borne pathogenic microorganisms comprises active groups including carboxy groups, carbonyl groups, hydroxy groups, epoxy groups and nitrogen-containing groups.
  • 专利号:   CN113173576-A, CN113173576-B
  • 发明人:   SUN D, YANG D, LI J, YIN J, ZHOU S, JIANG H, JIN M, SHI D, LI H, CHEN Z
  • 专利权人:   ENVIRONMENTAL MEDICINE OPERATIONAL MED
  • 国际专利分类:   C01B032/184, C12Q001/24
  • 专利详细信息:   CN113173576-A 27 Jul 2021 C01B-032/184 202170 Pages: 26 Chinese
  • 申请详细信息:   CN113173576-A CN10495083 07 May 2021
  • 优先权号:   CN10495083

▎ 摘  要

NOVELTY - Graphene aerogel comprises active groups including carboxy groups, carbonyl groups, hydroxy groups, epoxy groups and nitrogen-containing groups. USE - The graphene aerogel is useful in enrichment of food-borne pathogenic microorganisms. ADVANTAGE - The graphene aerogel has large specific surface area and three-dimensional network structure; is rich in nitrogen-containing active groups; and can efficiently adsorb and enrich food-borne pathogenic microorganisms in a sample. The elution method can efficiently elute the food-borne pathogenic microorganisms adsorbed on the graphene aerogel; and realizes enrichment and detection of the food-borne pathogenic microorganisms. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) preparing graphene aerogel comprising mixing graphene oxide with water and ultrasonically treating to obtain a graphene oxide dispersion, mixing graphene oxide dispersion with ammonia, triethylenetetramine and boric acid, performing oxidation-reduction reaction to obtain graphene hydrogel, washing graphene hydrogel and freeze-drying to obtain graphene aerogel; (2) use of graphene aerogel in enrichment of food-borne pathogenic microorganisms; and (3) eluting food-borne pathogenic microorganisms on graphene aerogel comprising placing the graphene aerogel adsorbing food-borne pathogenic microorganisms at the bottom of the syringe, eluting with an eluent and collecting the eluted solution, mixing the elution solution with the treatment reagent to obtain a liquid to be treated, allowing liquid to be treated to stand and centrifuging, fixing obtained centrifugal precipitate to volume with phosphate buffer solution and taking out the supernatant for bacterial culture or polymerase chain reaction, where the pH value of the eluent is 9, eluent is aqueous solution comprises 15 g/l sodium chloride, 20 g/l sodium hydroxide, 38.5 g/l glycine, 15 g/l beef powder, 30 g/l peptone, 34 g/l Tris-Base and 58.7 g/l 3-(N-morpholino)propanesulfonic acid (MOPS), treatment reagent is polyethylene glycol-6000 or Polyethylene glycol-8000 and dosage ratio of the elution solution to the treatment reagent is 10:1 (ml: g).