• 专利标题:   Optimizing loop-mediated isothermal amplification reaction useful for food safety detection, comprises adding graphene quantum dots into loop-mediated isothermal amplification reaction, and subjecting to turbidity and chromogenic detection.
  • 专利号:   CN110358811-A
  • 发明人:   LI X, JIN C
  • 专利权人:   SHANGHAI NAT ENG RES CENT NANOTECHNOLOGY
  • 国际专利分类:   C12Q001/6844
  • 专利详细信息:   CN110358811-A 22 Oct 2019 C12Q-001/6844 201988 Pages: 7 Chinese
  • 申请详细信息:   CN110358811-A CN10767206 20 Aug 2019
  • 优先权号:   CN10767206

▎ 摘  要

NOVELTY - Optimizing loop-mediated isothermal amplification reaction comprises (i) adding graphene quantum dots into the loop-mediated isothermal amplification reaction system and performing nucleic acid amplification; and (ii) detecting nucleic acid amplification product by electrophoresis, and subjecting to turbidity or chromogenic detection. USE - The method is useful for food safety detection. ADVANTAGE - The method increases the reaction sensitivity, reduces non-specific amplification and improves detection efficiency.