▎ 摘　　要

NOVELTY - A poly(N-isopropylacrylamide)-modified graphene quantum dot chromatographic filler comprises silicon spheres, graphene quantum dots and poly(N-isopropylacrylamide)bonded on the surface of the silicon spheres. USE - Poly(N-isopropylacrylamide)-modified graphene quantum dot chromatographic filler is used as stationary phase in high-performance liquid chromatography column for rapid detection/separation of hydrophilic substances e.g. nucleoside, nucleoside base, water-soluble vitamin and amino acid substance, and reverse phase chromatographic separation of benzene, biphenyl, polycyclic aromatic hydrocarbon, aniline and phenol with different numbers of carbon atoms substituted by alkyl chain (all claimed). ADVANTAGE - The poly(N-isopropylacrylamide)-modified graphene quantum dot chromatographic filler has high mechanical strength and separation selectivity, excellent physical and chemical properties, and rapid separation speed, and improves separation efficiency of the high-performance liquid chromatography column. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for preparation of the chromatographic filler, which involves amidating graphene oxide quantum dots on the surface of the silicon spheres bonded with a silanization reagent, generating an amide-based bonding material, which is the silicon spheres bonded with the graphene quantum dots, esterifying reversible addition-fragmentation chain transfer polymerization (RAFT) reagent on the surface of the silicon spheres, and combining N-isopropylacrylamide with silicon spheres bonded with graphene quantum dots and RAFT reagent.