▎ 摘　　要

NOVELTY - Experimental method of targeting graphene oxide complex comprises implanting experimental cell, animal and tumor xenotransplantation, MG63/Dox cells under the skin of the back of the mouse, and using the tumor-bearing mice for in vivo imaging and phototherapy, PPG, TPP-PPG and TPP-PPG-ICG preparation, (ii) characterizing optical properties of TPP-PPG, ICG and TPP-PPG-ICG by ultraviolet-visible-near-infrared spectrophotometer, singlet oxygen detection, (iii) using singlet oxygen green fluorescent probe to detect the generation of singlet oxygen, light-to-heat conversion experiment, irradiating with near-infrared light, using infrared thermometer to record temperature changes, cell uptake and intracellular localization, after culturing and incubating MG63/Dox cells, washing with phosphate buffered saline and resuspend in the culture medium, measuring the average fluorescence intensity of the sample by flow cytometry. USE - The complex is useful for treating drug-resistant osteosarcoma under the guidance of fluorescence imaging with coordinated phototherapy. ADVANTAGE - The complex ensures the sub-array will not be clamped by too inclined in the mounting process, realizes the radar sub-array in the guide rail gap is small, is suitable for maintenance and replacement, improves the installation success rate and efficiency of the radar sub-array, improves the radar sub-array maintenance efficiency, has easy operation, high efficiency and light weight, and is convenient to carry, durable, and high reliability. DETAILED DESCRIPTION - Experimental method of targeting graphene oxide complex comprises (i) implanting experimental cell, animal and tumor xenotransplantation, MG63/Dox cells under the skin of the back of the mouse, and using the tumor-bearing mice for in vivo imaging and phototherapy, PPG, TPP-PPG and TPP-PPG-ICG preparation, (ii) characterizing optical properties of TPP-PPG, ICG and TPP-PPG-ICG by ultraviolet-visible-near-infrared spectrophotometer, singlet oxygen detection, (iii) using singlet oxygen green fluorescent probe to detect the generation of singlet oxygen, light-to-heat conversion experiment, irradiating with near-infrared light, using infrared thermometer to record temperature changes, cell uptake and intracellular localization, after culturing and incubating MG63/Dox cells, washing with phosphate buffered saline and resuspend in the culture medium, measuring the average fluorescence intensity of the sample by flow cytometry, MG63/Dox cells co-cultured with TPP-PPG-ICG, washing the cells with phosphate buffered saline and incubating with mitochondrial fluorescent probes to label the mitochondria, washing cells again with phosphate buffered saline and scanned with a laser, focusing microscope scans to acquire images, in vitro experiments, culturing MG63/Dox cell suspension with different concentrations of ICG, TPP-PPG and PPG-ICG solutions, washing with phosphate buffered saline in the non-irradiated group and re-culturing, and the irradiated group irradiated with 808 nm NIR and re-culturing, detecting the cell viability at the same time for live cell/dead cell double staining, adding MG63/Dox cells to phosphate buffered saline, TPP-PPG-ICG, ICG, TPP-PPG, PPG-ICG and TPP-PPG-ICG culture, adding double staining kit and incubating using near infrared observe the fluorescence spectrum, detection of apoptosis and intracellular ROS, MG63/Dox cell culture, NIR irradiation, low-speed centrifugation to collect cells, resuspend in buffer to avoid light staining, low-speed centrifugation to collect cells, washing with phosphate buffered saline and dilute with binding buffer for flow cytometry analyze, using DCFH-DA kit to detect intracellular ROS production, detection of mitochondrial membrane potential, recording the changes of mitochondrial membrane potential with JC-1, and collecting images with a laser confocal microscope, detection of mitochondrial superoxide, using mitochondrial superoxide fluorescent probe to detect the production of mitochondrial superoxide, ATP detection, using ATP detection kit to draw ATP concentration standard curve, near-infrared fluorescence and thermal imaging, TPP-PPG-ICG was injected into the tail vein of MG63/Dox tumor-bearing nude mice, using the near-infrared imaging system for in vitro near-infrared imaging, when NRI is used for irradiation, the infrared thermal imaging camera is used to image the temperature changes of mouse tumor tissue in real time, in vivo PDT/PTT combination therapy, the tumor-bearing mice were randomly divided into 6 groups, phosphate buffered saline non-irradiated group (control group), TPP-PPG-ICG non-irradiated group, ICG irradiation group, TPP-PPG irradiation group, injecting in the PPG-ICG irradiation group and TPP-PPG-ICG irradiation group, ICG intravenously, and irradiating the irradiated group with 808 nm NIR at the tumor site for 15 days.