▎ 摘　　要

NOVELTY - Method for constructing electrochemical luminescence sensor, involves applying nitrogen-doped graphene-like carbon nanosheets and chitosan to surface of glassy carbon electrode, assembling carboxyl-modified capture DNA to electrode surface by an amide reaction, and obtaining capture DNA/graphene-like carbon nanosheets modified electrode. USE - The method is useful for constructing electrochemical luminescence sensor for detecting divalent mercury (claimed). DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for detecting divalent mercury, which involves taking capture DNA/nitrogen-doped graphene-like carbon nanosheets modified electrode as a working electrode, placing working electrode, reference electrode and counter electrode in a pyrosulfate solution, when working electrode is placed in silver nanocluster solution synthesized with T/C base-rich DNA as a template, linking silver nanoclusters to surface of capture DNA/nitrogen-doped graphene-like carbon nanosheets modified electrode by A-T base complementary hybridization, narrowing distance, attenuating electrochemiluminescence signal, when both silver nanoclusters and Hg2+ are present, forming T-Cg2+-T structure between template DNA rich in T/C bases or template DNA and Hg2+, attaching to surface of modified electrode, where if electrochemiluminescence signal is strong, and electrochemiluminescence signal intensity is linear with Hg2+ concentration, and constructing electrochemiluminescence sensor for sensitive detection of Hg2+.