▎ 摘　　要

NOVELTY - Method for detecting biomarker involves (a) separating nucleic acid from sample, (b) selecting a target nucleic acid of a biomarker by pretreating the nucleic acid, (c) preparing a fluorescent nucleic acid nanostructure-graphene oxide complex, and (d) (i) detecting the target nucleic acid, or (ii) reacting selected target nucleic acid with the fluorescent nucleic acid nanostructure-graphene oxide complex to detect the target nucleic acid. USE - The method is useful for detecting biomarker, and diagnosing a disease. ADVANTAGE - The method minimizes non-specific binding between target nucleic acid and fluorescent nucleic acid nanostructure-graphene structure, diagnoses multiple biomarker even contained in low concentration, and ensures early diagnosis of disease from liquid biopsy. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a biomarker detection kit, which comprises (i) a forward primer comprising a restriction enzyme recognition sequence, specifically binding to a target nucleic acid in a biomarker, and a reverse primer in which biotin is bound to the 5' end, (ii) restriction enzyme, (iii) magnetic particles coated with streptavidin, (iv) fluorescent nucleic acid nanostructure, and (v) graphene oxide.