▎ 摘　　要

NOVELTY - Preparing Escherichia coli graphene quantum dot immunofluorescence probe solution comprises dissolving bovine serum albumin, 1-ethyl-3-(3-dimethylamino) propyl carbodiimide, and N-hydroxysuccinimide in phosphate buffered saline having pH value of 7.4, adding phosphate buffered saline solution containing1-ethyl-3-(3-dimethylamino) propyl carbodiimide solution to graphene quantum dots, carrying out reaction, dropwise adding phosphate buffered saline solution containing N-hydroxysuccinimide into graphene quantum dots solution, adding phosphate buffer saline, to obtain solution A. USE - The method is useful for preparing Escherichia coli graphene quantum dot immunofluorescence probe solution (claimed). ADVANTAGE - The method has simple operation, consumes less time, good repeatability, high sensitivity, good specificity, less equipment requirement. DETAILED DESCRIPTION - Preparing Escherichia coli graphene quantum dot immunofluorescence probe solution comprises (i) dissolving bovine serum albumin, 1-ethyl-3-(3-dimethylamino) propyl carbodiimide, and N-hydroxysuccinimide (NHS) in 2-6 ml phosphate buffered saline (0.01 M) having pH value of 7.4, to obtain phosphate buffered saline solution containing1-ethyl-3-(3-dimethylamino) propyl carbodiimide, and N-hydroxysuccinimide (NHS); (ii) adding phosphate buffered saline solution containing1-ethyl-3-(3-dimethylamino) propyl carbodiimide solution to graphene quantum dots, carrying out reaction, to obtain graphene quantum dots solution; (iii) dropwise adding phosphate buffered saline solution containing N-hydroxysuccinimide into graphene quantum dots solution, adding phosphate buffer saline, to obtain solution A; (iv) dissolving Escherichia coli antibody in phosphate buffered saline buffer, to obtain solution B; and (v) uniformly mixing solution A and solution B, adding bovine serum albumin to phosphate buffered saline solution, carrying out reaction, to obtain quantum dot immunofluorescence probe solution C. An INDEPENDENT CLAIM is also included for determining Escherichia coli graphene quantum dot immunofluorescence probe solution comprising (A) uniformly mixing porphyrin quantum dot immunofluorescence probe, bacteria, to obtain probe-bacteria mixed liquid; (B) removing incubated probe-bacteria mixed liquid using micro-pipette, dripping on a glass slide under sterile environment, carrying out air-drying, covering using coverslip, to obtain slide with sample; (C) placing the slides with oil mirror on fluorescence microscopy, observing through ultraviolet light or blue light, green light, detecting sample on the slide through sample whether there is fluorescence, determining the fluorescence intensity and bacterial morphology, determining whether sample containing Escherichia coli O157:H7 content.