▎ 摘　　要

NOVELTY - This utility model claims a kind of a graphene nano-hole-micro-solid nano-pore structure when the DNA sequencing device, the device is mainly composed of a graphene strip with graphene nanometre hole, silicon substrate reverse pyramid micro solid nano-pore structure of micro-cavity top part. A sequencing, sequencing reaction chamber is graphite nano-hole-micro-solid nano-pore structure, single-stranded DNA molecule to pass through linear graphene nano hole under the action of the electrostatic field, the inverted pyramidal micro-cavity, at last passes through solid nanometre hole, using weak current measuring device at the same time test DNA molecule in a hole caused by vertical ion current blocking and graphene micro-nano hole around horizontal conductivity change, so as to utilize a synchronous recording and processing system for analysis calculation of data to realize the single chain DNA molecule of sequence analysis.