▎ 摘　　要

NOVELTY - The nanodevice comprises a reservoir separated into two parts by a graphene membrane, and a nanopore (106) formed through the graphene membrane. The nanopore connects the two parts of the reservoir. The graphene membrane is distinct from and physically separated from electrodes transferring current. The nanopore and the two parts of the reservoir are filled with an ionic buffer. The graphene membrane comprises a graphene layer or a graphene oxide layer (105) at an inner surface in the nanopore. USE - The nanodevice is useful as a chip for sequencing and sensing biomolecules such as protein, DNA and RNA. ADVANTAGE - The nanodevice is capable of rapidly sequencing and sensing biomolecules in an economic manner with single molecule accuracy and high spatial resolution. DETAILED DESCRIPTION - The nanodevice comprises a reservoir separated into two parts by a graphene membrane, and a nanopore (106) formed through the graphene membrane. The nanopore connects the two parts of the reservoir. The graphene membrane is distinct from and physically separated from electrodes transferring current. The nanopore and the two parts of the reservoir are filled with an ionic buffer. The graphene membrane comprises a graphene layer or a graphene oxide layer (105) at an inner surface in the nanopore. The graphene layer or graphene oxide layer is coated with an organic layer configured to interact with biomolecules in a different way in order to differentiate the biomolecules or bases of the molecule. The organic layer enhances resolution and motion control of the biomolecules. A time trace of ionic current is monitored to identify the biomolecules or the bases of the molecule based on a respective interaction of the bases or biomolecules with the organic layer. The time trace of the ionic current for each of the biomolecules or bases comprises a magnitude of the ionic current and a duration in time of the ionic current. The ionic current is generated through the nanopore when a voltage is applied. The organic layer has amine functionality to bond to carboxyl groups of the graphene oxide layer. The ionic current through the nanopore changes for each of the biomolecules to identify types of the biomolecules or bases based on both a magnitude of the ionic current and a duration in time of the ionic current while an individual one of the biomolecules or bases is provided in the nanopore. The biomolecules comprise a first biomolecule, a second biomolecule, and a third biomolecule. The organic layer is configured to bond to the first biomolecule stronger than the second and third biomolecules which causes the first biomolecule to remain longer in the nanopore than the second and third biomolecules. The organic layer bonding stronger to the first biomolecule causes the first biomolecule to have a longer duration in time for the ionic current resulting from remaining longer in the nanopore. A pair for the first biomolecule and the organic layer is respectively an antigen and an antibody pair. The graphene oxide layer has a thickness of 0.3 nanometers. The graphene layer is oxidized to graphene oxide, where the graphene oxide at an inner surface of the nanopore is coated with the organic layer. DESCRIPTION OF DRAWING(S) - The diagram shows a schematic cross-sectional view of a fabrication process of a graphene nanopore device. Substrate (101) Hole (104) Graphene layer and/or graphene oxide layer (105) Nanopore (106) Organic coating. (107)