▎ 摘　　要

NOVELTY - Cell dynamic monitoring system based on three-dimensional graphene interface electrode comprises a microfluidic chip, a PCB board, an impedance meter, an injection system and an inverted microscope with a CCD. The microfluidic chip comprises an H-shaped PDMS flow channel formed by two reservoirs, a test flow channel located between the two reservoirs and a sub-element arranged in the test flow channel chip. The each of the two reservoirs and the test flow channel are provided with a cover plate. USE - Used as cell dynamic monitoring system based on three-dimensional graphene interface electrode. ADVANTAGE - The system improves the single cell sensing performance by an average of 100% and the double cell sensing performance by an average of 50%. DETAILED DESCRIPTION - Cell dynamic monitoring system based on three-dimensional graphene interface electrode comprises a microfluidic chip, a PCB board, an impedance meter, an injection system and an inverted microscope with a CCD. The microfluidic chip comprises an H-shaped PDMS flow channel formed by two reservoirs, a test flow channel located between the two reservoirs and a sub-element arranged in the test flow channel chip. The each of the two reservoirs and the test flow channel are provided with a cover plate. The tank wall of the sump or the cover plate sealing the sump has an opening, where an opening is connected with the injection system. The sub-chip includes a center reference electrode disposed on an axis of symmetry and atleast one independent cell electrodes symmetrically arranged on two sides of the center reference electrode. The cell electrodes on both sides are captured in opposite directions and perpendicular to the central reference electrode. The one side constitutes the working electrode and the other side constitutes the counter electrode. The working electrode, the counter electrode and the reference electrode are respectively connected with the impedance meter, and the impedance meter outputs an electrical signal to the reference electrode for applying the electric field. The working cell and the cell signal collected from the counter electrode are obtained. The cell electrode comprises an electrode base and a catching groove on the electrode base. The catching groove comprises of a plurality of microelectrodes of the gap. The plurality of microelectrodes is arranged in an orderly manner to form an arc-shaped capturing surface perpendicular to the electrode base. The arcs of which are semi-ellipses divided along the short axis. The electrode base comprises a gold layer and a metal layer on the surface of the graphene layer. The graphene layer is provided with micro-nano folds and textured structures that match the filopodia of the cell surface. The gold layer in the electrode base is connected to the wiring terminals arranged on the edge of the chip by lead wires to connect the impedance meter of the inverted microscope lens aligned sub-chip. An INDEPENDENT CLAIM is also included for the real-time monitoring of cancer cell dynamic characteristics using the system, comprising, (i) injecting cytological fluid into one of the liquid reservoirs through an injection pump, flowing the cytological fluid through the test fluid channel, partially capturing the trapped fluid and flowing the other fluid to the other fluid reservoir slot through the openings, (ii) generating an AC signal with a magnitude of 0.5V and a frequency of 5k and 10kHz with a sampling rate of 220/seconds for time-domain testing or a 500mVP amplitude with a frequency of 100-106Hz sinusoidal signal for frequency domain testing by a impedance meter, (iii taking one side of the trapping cell as the working electrode and the other side as the counter electrode, obtaining the impedance signals of the working electrode and the counter electrode in real time by two channels of the impedance meter respectively and obtaining the electrical impedance signal of single cell/multi-cell physiological activity by subtracting the impedance value of the counter electrode from the working electrode impedance value and (iv) collecting the electrical impedance signal, where the inverted microscope objective goes deep into the microfluidic channel and recording the CCD real-time changes in cell surface morphology.