▎ 摘　　要

NOVELTY - Culture scaffold comprises multi-layer graphene film. USE - The culture scaffold is useful in pharmaceutical composition for promoting stem cell differentiation and bone differentiation of stem cell and treating bone disease. The stem cell is chosen from embryonic stem cells, and adult stem cells. The adult stem cells are mesenchymal stem cells, hematopoietic stem cells, adipose derived stem cells or neural stem cells (all claimed). ADVANTAGE - The culture scaffold promotes bone differentiation without using differentiation-inducing substance, and carbon harmful to human body and reduces side effects. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) method for regulating growth and differentiation of stem cells, involves culturing the stem cells on the culture support comprising multilayer graphene film; and (2) method for producing a culture scaffold, involves synthesizing a monolayer graphene film, and laminating the synthesized graphene film.