▎ 摘　　要

NOVELTY - Detecting human interleukin-6 through an electro-chemical luminous immune sensing agent, is claimed, where a surface of the sensing agent is covered with a graphene oxide nano-sheet containing glassy carbon electrode/polyaniline nano-wire array/cadmium selenide quantum point compound film that is formed by reacting 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide amine hydrochloride and N-hydroxy succinimide. USE - The method is useful for detecting human interleukin-6 through electro-chemical luminous immune sensing agent (claimed). ADVANTAGE - The method is capable of simply and effectively detecting human interleukin-6 through the electrochemical luminous immune sensing agent with high sensitivity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for preparing the electro-chemical luminous immune sensing agent for detecting human interleukin-6 comprising adding 50 mu l of aniline in 20 ml of concentrated sulfuric acid solution, ultrasonic processing for 0.5 hours to obtain the aniline sulfuric acid solution, mixing 20 ml of aniline sulfuric acid solution and 0.5 mg/ml of graphene oxide solution at room temperature stirring for 30 minutes, adding 0.1512 g of ammonium persulfate, continuously stirring for 12 hours, centrifugally washing with de-ionized water at pH of 6-7, vacuum drying at 65 degrees C to obtain an atrovirens product, dissolving 0.5 mg/ml of nano graphene oxide/polyaniline nanowire array in deionized water as solvent to obtain nano graphene oxide/polyaniline nano-wire array complex solution, dissolving 0.5 mg/ml nano graphene/polyaniline nano-wire array complex solution in deionized water as solvent to obtain a quantum dot complex solution, drying the quantum dot complex solution, ultrasonic cleaning, and drying to obtain the glassy carbon electrode having median particle diameter of 1 microns, preparing a nano graphene oxide/polyaniline nano-wire array/cadmium selenide quantum point compound solution at room temperature to form a nano graphene oxide/polyaniline nano-wire array/cadmium selenide quantum dot complex film, then adding 10 mu l of 1-3-(3- amino propyl) carbodiimide hydrochloride and 10-20 mg/ml of N-hydroxysuccinimide mixed solution into the nano graphene oxide/polyaniline nanowire array/cadmium selenide quantum point compound film, standing for 20-30 minutes to obtain a sample, adding 0.1 mg/ml of phosphate buffer solution as solvent into the human interleukin-6 antibody for 12-20 hours at pH of 7.4, taking out the phosphate buffer solution to wash, adding 2-3% of bovine serum albumin for 1-2 hours, taking out and cleaning to obtain the human interleukin-6 electro-chemical luminous immunity sensing agent.