▎ 摘　　要

NOVELTY - Preparation of graphene tube electro-chromatography column involves washing capillary tube using sodium hydroxide, drying, adding N-phenyl-3-aminopropyl trimethoxy silane-ethanol solution in capillary tube, drying, respectively disposing hydrochloric acid solution of aniline and hydrochloric acid solution of ammonium sulfate, mixing the solutions in capillary tube, reacting, injecting dopamine, drying, obtaining modified inner wall coated with dopamine capillary tube, feeding aqueous solution of graphene oxide having concentration of 1 mg/mL into capillary tube, reacting, and drying. USE - Preparation of graphene tube electro-chromatography column (claimed). ADVANTAGE - The method economically and environmentally-friendly provides graphene tube electro-chromatography column having favorable stability and long-term durability, by a simple process. DETAILED DESCRIPTION - Preparation of graphene tube electro-chromatography column involves washing capillary tube using 1 m sodium hydroxide for 2 hours, then using pure water for half an hour, drying under nitrogen, adding N-phenyl-3-aminopropyl trimethoxy silane-ethanol solution adding N-3-amino propyl trimethoxy silane ethanol solution obtained by by carrying out silylation reaction at 60 degrees C for 12-24 hours in the capillary tube, washing using pure ethanol, drying under nitrogen, respectively disposing 0.2 M hydrochloric acid solution of aniline and 0.05M hydrochloric acid solution of ammonium sulfate, mixing the solutions in the capillary tube, reacting for overnight, washing using pure water, processing at 120 degrees C for 3 hours, injecting dopamine at a flow speed of 0.05 mL/hour for 8-16 hours, washing using pure water, drying under nitrogen, obtaining modified inner wall coated with dopamine capillary tube, feeding aqueous solution of graphene oxide having concentration of 1 mg/mL into capillary tube, reacting at 60 degrees C under water bath for 12-24 hours, and washing using pure water. 10 mM Tris-hydrochloric acid and buffer solution with pH of 8.5 are reacted, to obtain dopamine having concentration of 2 mg/mL.