▎ 摘　　要

NOVELTY - Detecting copper ions based on template-dependent click chemistry and graphene oxide, involves (a) dissolving the short single-stranded nucleic acid modified with azide group, the short single-stranded nucleic acid modified with alkynyl group, and the long single-stranded nucleic acid modified with fluorescent group as the connection template in pure water respectively preparing into a nucleic acid solution, (b) mixing the three nucleic acid solutions to prepare a mixed solution, then adding the test sample, ascorbic acid, water and Tris hydrochloride (HCl) buffer solution to the mixed solution to form a reaction system, reacting for a certain period of time, and the test sample contains reagents with known concentrations of copper ions, and (c) adding water, graphene oxide and Tris-HCl buffer solution to the reaction system, and detecting the fluorescence intensity of the detection system after heating up. USE - Method for detecting copper ions based on template-dependent click chemistry and graphene oxide. ADVANTAGE - The detection method has good stability high sensitivity strong anti-interference ability with good response relation in the range of 0 -900 nM detection limit is low to 0 25 nM. DETAILED DESCRIPTION - Detecting copper ions based on template-dependent click chemistry and graphene oxide, involves (a) dissolving the short single-stranded nucleic acid modified with azide group, the short single-stranded nucleic acid modified with alkynyl group, and the long single-stranded nucleic acid modified with fluorescent group as the connection template in pure water respectively preparing into a nucleic acid solution, (b) mixing the three nucleic acid solutions to prepare a mixed solution, then add the test sample, ascorbic acid, water and Tris hydrochloride (HCl) buffer solution to the mixed solution to form a reaction system, react for a certain period of time, and the test sample contains reagents with known concentrations of copper ions, (c) adding water, grarphene oxide and Tris-HCl buffer solution to the reaction system, and detecting the fluorescence intensity of the detection system after heating up, and (d) taking the known copper ion concentration as the abscissa and the corresponding fluorescence intensity value, and drawing a standard curve to obtain the linear relationship equation between the copper ion concentration and the fluorescence intensity, and then calculating the concentration of copper ions in the corresponding test sample according to the fluorescence intensity of the sample to be tested. The nucleic acid used as the ligation template is selected from a nucleic acid chain marked with a fluorescent group.