• 专利标题:   New single-stranded DNA aptamer useful for detecting N-acetylneuraminic acid.
  • 专利号:   CN113151283-A, CN113151283-B
  • 发明人:   ZHOU N, YUE H, ZHANG Y, WANG X
  • 专利权人:   UNIV JIANGNAN
  • 国际专利分类:   C12N015/115, G01N021/64, G01N033/53
  • 专利详细信息:   CN113151283-A 23 Jul 2021 C12N-015/115 202168 Pages: 11 Chinese
  • 申请详细信息:   CN113151283-A CN10206562 24 Feb 2021
  • 优先权号:   CN10206562

▎ 摘  要

NOVELTY - Single-stranded DNA (ssDNA) aptamer, comprising a fully defined sequence of 79 nucleotides (SEQ ID NO: 1), a sequence of SEQ ID NO: 2, a fully defined sequence of 79 nucleotides (SEQ ID NO: 3), a fully defined sequence of 79 nucleotides (SEQ ID NO: 4) and a fully defined sequence of 79 nucleotides (SEQ ID NO: 5), as given in the specification, is new. USE - The ssDNA aptamer is useful for detecting N-acetylneuraminic acid (claimed). ADVANTAGE - The aptamer: has high binding specificity and affinity to N-acetylneuraminic acid. DETAILED DESCRIPTION - Single-stranded DNA (ssDNA) aptamer, comprising a fully defined sequence of 79 nucleotides (SEQ ID NO: 1), 5'ggaaaagaatttatcttgtcc3' (SEQ ID NO: 2), a fully defined sequence of 79 nucleotides (SEQ ID NO: 3), a fully defined sequence of 79 nucleotides (SEQ ID NO: 4) and a fully defined sequence of 79 nucleotides (SEQ ID NO: 5), as given in the specification, is new INDEPENDENT CLAIMS are also included for: (1) a composition for detecting N-acetylneuraminic acid, comprising the ssDNA aptamer; (2) a kit for detecting N-acetylneuraminic acid, comprising the ssDNA aptamer; (3) a test paper for detecting N-acetylneuraminic acid, comprising the ssDNA aptamer; (4) a chip for detecting N-acetylneuraminic acid, comprising the ssDNA aptamer; and (5) a fluorescent biosensor for detecting N-acetylneuraminic acid, comprising the ssDNA aptamer, a fluorescent group and a fluorescent quenching agent.