▎ 摘　　要

NOVELTY - Biosensor 1 comprises an electrical transistor transducer 3 comprising a transistor gate electrode, a sensing electrode element comprising a metal element having a biofluid facing surface, and a graphene layer 23 on the biofluid facing surface of metal element. The sensing electrode element is connected with the transistor gate electrode by an electrical connector to form an extended gate configuration with the transistor gate electrode. The biosensor comprises a reference electrode 29 configured to be immersed in, or in contact with the biofluid, and is configured to electrically bias the transistor gate electrode through the biofluid. The sensing electrode element is functionalized by at least a layer of aptamers 25 placed indirectly or directly on the graphene layer, and is configured to catch hormones in the biofluid to change a surface potential of the sensing electrode element. USE - Biosensor for sensing hormone concentration in biofluid and used in wearable lab-on-chip device (claimed) and for sensing stress hormone cortisol concentration in a biofluid. ADVANTAGE - The biosensor senses concentration of hormone of stress and cortisol in human biofluids, such as blood, sweat, interstitial fluids, saliva, tears and urine. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for fabricating a biosensor for sensing hormone concentration in a biofluid by depositing a layer of graphene on a first layer of metal; coating layer of graphene with a layer or thermoplastic; removing first layer or metal; transferring layer of graphene together with the layer of thermoplastic onto a second layer of metal; removing the layer of thermoplastic; functionalizing the layer of graphene with a layer of aptamers to form a functionalized sensing electrode element; connecting the sensing electrode element to a transistor gate electrode of an electrical transistor transducer by an electrical connector to form an extended gate configuration with the transistor gate electrode; and adding a reference electrode configured to be immersed in, or in contact with the biofluid, and is configured to electrically bias the transistor gate electrode through the biofluid. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of biosensor. Biosensor (1) Electrical transistor transducer (3) Graphene layer (23) Aptamer (25) Reference electrode (29)