▎ 摘　　要

NOVELTY - A dopamine detection method involves adding citric acid and urea to hydrothermal kettle, heating and taking out obtained product. The obtained product is washed with ethanol for 3 times followed by centrifuging to remove impurities and obtaining 20 mg/l nitrogen-doped graphene quantum dot solution. The nitrogen-doped graphene quantum dot solution is added to 0.1 M, 1.2 mu g/l phosphate buffer solution (pH 7.4) followed by measuring fluorescence spectrum of obtained solution, measuring initial fluorescence intensity (F0), adding dopamine solution with certain concentration, obtaining standard solution with standard dopamine concentrations (0-4000 nM/l), recording fluorescence intensity (F), establishing standard curve for fluorescence quenching intensity of concentration of dopamine with graphene quantum dots, measuring fluorescence intensity of to-be-detected sample and calculating dopamine concentration. USE - Method for detecting dopamine. ADVANTAGE - The method enables detecting dopamine content in blood and drugs with less interference and linear range of detection of 0.05-4000 nM in a simple and rapid manner. DETAILED DESCRIPTION - A dopamine detection method involves adding citric acid and urea in a molar ratio of 1:3.05 to hydrothermal kettle, heating at 180 degrees C for 8 hours and taking out obtained product. The obtained product is washed with ethanol for 3 times followed by centrifuging to remove impurities and obtaining 20 mg/l nitrogen-doped graphene quantum dot solution. The nitrogen-doped graphene quantum dot solution is added to 0.1 M, 1.2 mu g/l phosphate buffer solution (pH 7.4) followed by measuring fluorescence spectrum of obtained solution, measuring initial fluorescence intensity (F0), adding dopamine solution with certain concentration, obtaining standard solution with standard dopamine concentrations (0-4000 nM/l), recording fluorescence intensity (F), establishing standard curve for fluorescence quenching intensity of concentration of dopamine with graphene quantum dots, measuring fluorescence intensity of to-be-detected sample and calculating dopamine concentration, where the fluorescence quenching intensity is represented as (F0-F) divide by F0.