• 专利标题:   Droplet digital PCR system with graphene heating comprises graphene heating sheet, droplet digital PCR microfluidic chip, liquid inlet system, temperature measurement and control system and fluorescence detection image acquisition system.
  • 专利号:   CN111235007-A
  • 发明人:   LI S, JIANG Y, FU H, PENG J
  • 专利权人:   HARBIN INST TECHNOLOGY
  • 国际专利分类:   C12M001/00, C12M001/34, C12M001/38
  • 专利详细信息:   CN111235007-A 05 Jun 2020 C12M-001/00 202049 Pages: 10 Chinese
  • 申请详细信息:   CN111235007-A CN10113427 24 Feb 2020
  • 优先权号:   CN10113427

▎ 摘  要

NOVELTY - Droplet digital PCR system with graphene heating comprises a graphene heating sheet (5), a droplet digital PCR microfluidic chip (3), a liquid inlet system (4), a temperature measurement and control system (1) and fluorescence detection image acquisition system (2). The droplet digital PCR microfluidic chip includes a PDMS chip and a glass sheet. The bottom surface of the PDMS chip is etched with channels. The bottom surface of the PDMS chip is bonded to one surface of the glass sheet to form a flow channel. The flow channel includes a liquid inlet, a liquid inlet channel, a reaction channel and a liquid outlet. The liquid inlet, the liquid inlet flow channel, the reaction flow channel and the liquid outlet are sequentially connected. The liquid inlet flow channel is used for condensing the two-phase fluid input from the liquid inlet system to generate a PCR reaction liquid, and the PCR reaction liquid is in the form of droplets in the reaction flow channel. USE - Used as a droplet digital PCR system with graphene heating. ADVANTAGE - The system shortens amplification time. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic representation of the droplet digital PCR system with graphene heating (Drawing includes non-English language text). Temperature measurement and control system (1) Fluorescence detection image acquisition system (2) Droplet digital PCR microfluidic chip (3) Liquid inlet system (4) Graphene heating sheet (5)