▎ 摘　　要

NOVELTY - Method of nucleic acid target detection and/or quantification by nucleic acid amplification comprises: providing at least one target nucleic acid, at least one oligonucleotide non-extendable or extendable, labelled with at least one shining group/moiety adapted to shine extra upon hybridization or getting incorporated into a nucleic acid molecule; providing at least one extendable oligonucleotide labelled with at least one converter or acceptor group/moiety adapted to convert the color of the shining group/moiety to a different color or thermalise it, is incorporated into an amplification product; and hybridizing or incorporating into a target nucleic acid amplification product by shining group/moiety of the shining group labelled oligonucleotide. USE - The method is useful for nucleic acid target detection and quantification by nucleic acid amplification. ADVANTAGE - The target nucleic acid has very high specificity and sensitivity. DETAILED DESCRIPTION - Method of nucleic acid target detection and/or quantification by nucleic acid amplification comprises: providing at least one target nucleic acid, at least one oligonucleotide non-extendable or extendable, labelled with at least one shining group/moiety adapted to shine extra upon hybridization or getting incorporated into a nucleic acid molecule; providing at least one extendable oligonucleotide labelled with at least one converter or acceptor group/moiety adapted to convert the color of the shining group/moiety to a different color or thermalise it, is incorporated into an amplification product; and hybridizing or incorporating into a target nucleic acid amplification product by shining group/moiety of the shining group labelled oligonucleotide where the shining group/moiety shines extra with the extra shine is a measure of the target amplification; where the nucleic acid amplification is carried out such that any non-specific amplification does not generate any detectable signal under controlled attenuation by selectively controlling the removal of the extra shine of the shining group in the non-specific amplification product by the converter or acceptor group/moiety by selective positioning of the signaling moiety on signaling oligonucleotide and selective positioning of the attenuator or acceptor moiety on attenuator or acceptor oligonucleotide for a particular signaling moiety and attenuator/converter or acceptor moiety pair such that there is no net signal enhancement and no net signal from non-specific product, no net signal attenuation and no loss of target amplification signal. An INDEPENDENT CLAIM is also included for kit for carrying out nucleic acid amplification reaction, comprising at least one container, at least a donor fluorophore labelled oligonucleotide probe and acceptor fluorophore or quencher labelled oligonucleotide primers including (a) at least a donor fluorophore labelled oligonucleotide primer and an acceptor fluorophore or quencher labelled oligonucleotide primers, (b) at least donor fluorophore labelled oligonucleotide primer or primers and acceptor fluorophore or quencher labelled oligonucleotide primer or primers, (c) at least donor fluorophore labelled oligonucleotide probe or probes and acceptor fluorophore or quencher labelled oligonucleotide primer or primers, (d) at least donor fluorophore and acceptor fluorophore/quencher labelled promoter sequence carrying first primer and target specific second primer, (e) at least a positive control template and positive control template specific donor fluorophore and acceptor fluorophore/quencher labelled primer pair or primer and probe, and (f) labelled primer pair.