▎ 摘　　要

NOVELTY - Preparing graphene oxide-coated metal nano-materials with a SERS signal involves using Hummers method to prepare a graphene oxide solution, filtering through a 0.2-0.5 mu m filter, ultrasonically crushing for 0.5-5 hours. The supernatant is centrifuged to obtain a 50 plus minus 5nm graphene oxide solution. The 10-30 volume of 0.01-0.1 milligram per milliliter positively charged gold nanoparticle solution is centrifuged with a 0.5-3 volume of 0.5-5 millimole PATP aqueous solution after centrifugation, dispersed into Tris-HCl buffer solution with pH of 2-4 with mass percentage of 0.001-0.1% Tween (RTM: non ionic surfuctant), after reaction overnight, adding 0.1-1 volume of 0.1-0.5M CTAC solution, after centrifugation. The lower precipitate is re-dispersed to gold-p-aminothiophenol in 0.5-5mM CTAC solution. USE - Method for preparing graphene oxide-coated metal nano-materials with SERS signal used in fields of Raman detection and biological SERS imaging (claimed). ADVANTAGE - The method enables to prepare graphene oxide-coated metal nano-materials with a SERS signal is simple to prepare and has good application potential. DETAILED DESCRIPTION - Preparing graphene oxide-coated metal nano-materials with a SERS signal involves using Hummers method to prepare a graphene oxide solution, filtering through a 0.2-0.5 mu m filter, ultrasonically crushing for 0.5-5 hours. The supernatant is centrifuged to obtain a 50 plus minus 5nm graphene oxide solution. The 10-30 volume of 0.01-0.1 milligram per milliliter positively charged gold nanoparticle solution is centrifuged with a 0.5-3 volume of 0.5-5mM PATP aqueous solution after centrifugation, dispersed into Tris-HCl buffer solution with pH of 2-4 with mass percentage of 0.001-0.1% Tween (RTM: non ionic surfuctant), after reaction overnight, adding 0.1-1 volume of 0.1-0.5M CTAC solution, after centrifugation. The lower precipitate is re-dispersed to gold-p-aminothiophenol in 0.5-5mM CTAC solution. The 0.5-5 volumes of obtained gold-p-aminothiophenol and 0.5-5 volumes of obtained graphene oxide dispersion in 1-10 volumes of ultrapure water, and slowly stirred the reaction for 0.5-5 hours to prepare gold-p-aminothiophenol-graphene oxide materials or added 5-20 volumes of 5-20 millimole silver nitrate to the 500-2000 volume of gold-p-aminothiophenol, mix well, and then slowly added 500-2000 volumes of 0.5-5mM ascorbic acid at a rate of 1-10 milliliter per hour, after centrifugation, resuspending in ultrapure water to obtain gold-p-aminothiophenol-silver sol. The 0.5-5 volumes of the gold-p-aminothiophenol-silver sol obtained above and 0.5-5 volumes of the graphene oxide dispersion are mixed in 1-10 volumes of ultrapure water. The reaction is stirred slowly for 0.5-5 hours to prepare gold-p-aminothiophenol-silver-graphene oxide material.