▎ 摘　　要

NOVELTY - Three-dimensional biomimetic nanomaterial comprises porous graphene (2), silicon microsphere (3) and silicon base material (1). The surface of silicon base material is chemically modified. The E-selectin protein and recognition ligand are respectively immobilized on porous graphene and silicon microspheres, used for identification and capturing of circulating tumor cells. The gold nanoparticles are adsorbed on the porous graphene and silicon microspheres to form a gold array. The thickness of gold nanoparticle is 10-20 nanometer. USE - Three-dimensional biomimetic nanomaterial for capturing of circulating tumor cells, and for increasing separating efficiency. ADVANTAGE - The three-dimensional biomimetic nanomaterial is good for detection and analysis of captured cells, suitable for capturing different type of cells effectively, and identifies and captures rare tumor cells in peripheral blood sample. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for preparing three-dimensional biomimetic nanomaterial, which involves: (A) preparing porous graphene material, preparing a porous structure template, then chemically synthesizing the silicon base material; (B) forming a gold array on the silicon base material by using stripping method or an etching method, transferring porous graphene to silicon base material, then aligning porous graphene on gold array; (C) inducing the positive charges on the gold surface by using chemical polarization, adsorbing the silicon microsphere on the gold surface to obtain three-dimensional nanostructures, then modifying and treating the surface of the graphene and the surface of the silicon microspheres respectively; and (D) immobilizing the E-selectin protein molecules and tumor cell recognition ligands on the linker to prepare the three-dimensional biomimetic nanomaterials. DESCRIPTION OF DRAWING(S) - The drawing shows the schematic view of three-dimensional biomimetic nanomaterial. Silicon base material (1) Porous graphene (2) Silicon microsphere (3)